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Series GSE56917 Query DataSets for GSE56917
Status Public on Apr 16, 2015
Title Identification of the hypothalamic neuronal populations that derive from Pomc-expressing precursors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hypothalamic neuronal populations are central regulators of energy homeostasis and reproductive function. However, the ontogeny of these critical hypothalamic neuronal populations is largely unknown. Here, we reveal novel cellular fates of the hypothalamic Pomc-expressing precursors by combining mouse genetics with a conditional viral ribosome-tagging approach to phenotype neurons. Our results show that the Pomc-expressing precursors differentiate into discrete neuronal subpopulations that mediate not only energy balance (POMC and AgRP) but also reproductive physiology (Kisspeptin).
Overall design Punches containing the mediobasal hypothalamus of Pomc-Cre:RiboTag mice and Pomc-Cre mice injected with the RiboTag viral vector (AAV-DIO-RiboTag) were homogenized and incubated with anti-hemagglutin (HA) antibodies and protein A/G magnetic beads to isolate the polysome-associated mRNAS in the Pomc-derived lineage and in adult POMC neurons, respectively. To identify differentially expressed transcripts, RNA from the immunoprecipitates was extracted, amplified, labelled and hybridized to MouseRef-8 v2 expression beadchips.
Contributor(s) Sanz E, McKnight GS
Citation(s) 25855171
Submission date Apr 18, 2014
Last update date Jun 14, 2018
Contact name Elisenda Sanz
Organization name Universitat Autonoma de Barcelona
Department Institut de Neurociencies
Street address M1/113 Facultat de Medicina
City Bellaterra
State/province Barcelona
ZIP/Postal code 08193
Country Spain
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM1371152 IP Pomc-cre AAV-DIO-RiboTag_rep1
GSM1371153 IP Pomc-cre AAV-DIO-RiboTag_rep2
GSM1371154 IP Pomc-Cre:RiboTag mouse_rep1
BioProject PRJNA244931

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Supplementary file Size Download File type/resource
GSE56917_RAW.tar 3.1 Mb (http)(custom) TAR
GSE56917_non-normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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