|
| Status |
Public on Apr 21, 2015 |
| Title |
Somatic mutations in DROSHA and DICER1 impair microRNA biogenesis in Wilms tumors |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Through whole-exome sequencing we identified somatic missense mutations in DICER1 and DROSHA in Wilms tumor, a childhood kidney cancer. DICER1 and DROSHA are key enzymes in the microRNA biogenesis pathway. To determine the effect of these mutations on microRNA expression, we prepared small RNAs from Wilms tumors and used next-generation sequencing to determine the expression levels of microRNAs in the tumors.
|
| |
|
| Overall design |
Comparison of miRNA expression in tumors with and without mutations in DICER1 or DROSHA.
|
| |
|
| Contributor(s) |
Amatruda JF, Mendell JT |
| Citation(s) |
25190313 |
| Submission date |
Apr 22, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
James Francis Amatruda |
| Organization name |
UT Southwestern
|
| Department |
Pediatrics, Internal Medicine, and Molecular BIology
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16288 |
AB 5500xl Genetic Analyzer (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA245069 |
| SRA |
SRP041335 |
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