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| Status |
Public on May 15, 2014 |
| Title |
Global transcriptional profiles of WT and HOIL-1L-/- BMDMs in response to LPS and TNFa |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
HOIL-1L is an essential member of the linear ubiquitin assembly complex (LUBAC), which targets Nemo for linear ubiquitination during NF-kB activation in response to a variety of simuli, including LPS and TNFa treatment. HOIL-1L has also been suggested to function as a transcription factor.
Here we analyzed changes in the global transcriptional profiles of primary bone marrow derived macrophage (BMDMs) from WT and HOIL-1L-/- mice upon treatment with NF-kB activating simuli.
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| Overall design |
BMDMs were differentiated for 1 week and then treated with mock (untreated), 10ng/ml LPS, or 1ng/ml TNFa for 4 hours. RNA was immediately collected and analyzed in biological duplicates by the Agilent 8x60 mouse array.
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| Contributor(s) |
Rodgers MA, Jung JU |
| Citation(s) |
24958845 |
| Submission date |
Apr 30, 2014 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Mary A Rodgers |
| Phone |
323-442-1066
|
| Organization name |
University of Southern California
|
| Department |
Molecular Microbiology and Immunology
|
| Lab |
Dr Jae Jung
|
| Street address |
1450 Biggy St, NRT 5517
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA245855 |
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