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| Status |
Public on May 01, 2014 |
| Title |
Loss of Runx3 in osteoblasts provokes severe congenital osteopenia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Murine Runx3 is expressed in developing bone osteoblasts (OBLs) and its deletion in these cells culminates in severe congenital osteopenia. We demonstrate that Runx3 is non-redundantly involved in the proliferation of early pre-committed OBL progenitor cells, a critical step in the generation of adequate numbers of bone-forming OBLs. Thus, in the absence of Runx3 in cells of this lineage, the number of mature/active OBLs is significantly diminished, providing a mechanistic explanation to the observed osteopenia.
This experiment was aimed at identifying genes that are likely regulated by Runx3 along osteoblastogenesis.
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| Overall design |
To elucidate the Runx3-mediated transcriptional program along OBL differentiation, we compared gene expression profile in WT and Runx3f/f/Col1a1-cre calvarial OBLs. Progenitor cells were purified and RNA extracted, reverse-transcribed, fragmented, labeled and hybridized to GeneChip® Mouse Gene 2.0 ST Array.
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| Contributor(s) |
Bauer O, Sharir A, Kimura A, Hantisteanu S, Takeda S, Groner Y |
| Citation(s) |
25605327 |
| Submission date |
Apr 30, 2014 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Omri Bauer |
| E-mail(s) |
Omri.Bauer@weizmann.ac.il
|
| Phone |
972-8-9342318
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Molecular Genetics
|
| Lab |
Prof. Yoram Groner
|
| Street address |
Herzl
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| GSM1376973 |
Runx3f/f/Col1a1-cre primary OBLs, biological rep 1 |
| GSM1376974 |
WT primary OBLs, biological rep 1 |
| GSM1376975 |
Runx3f/f/Col1a1-cre primary OBLs, biological rep 2 |
|
| Relations |
| BioProject |
PRJNA245888 |
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