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Series GSE5723 Query DataSets for GSE5723
Status Public on Jan 08, 2007
Title IdentificationOf genes responsive to non-metabolised glucose analogs: approach to hexokinase-independent glucose sensing
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary It has been strongly argued that plant cells should have a means of sensing sugars at the cell surface, so that extracellular and intracellular sugars can be sensed separately and their metabolism coordinated (Lalonde et al., Plant Cell, 11, 707-26, 2000). There is good evidence for an intracellular hexokinase-dependent pathway of hexose sensing in plants, but very little evidence for a hexokinase-independent signalling pathway, such as that provided by SNF3 or RGT2 in yeast. Many papers on sugar sensing in plants cite work from two laboratories as evidence for hexokinase-independent hexose signalling in plants. The first is that in which cell-wall invertase and sucrose synthase genes were induced by treatment of a Chenopodium suspension culture with 30 mM 6-Deoxyglucose (6DOG) for 24 h (Roitsch et al., Plant Physiol 108, 285-294, 1995; Godt et al., J. Plant Physiol 146, 231-238, 1995). The second is that in which a patatin transgene in Arabidopsis was shown to be weakly induced by growth over several days on a mixture of 30 mM glucose plus 30 mM 3-O-methylglucose (3OMG), but strongly induced by growth on 30 mM Glc plus 90 mM 3OMG (Martin et al., Plant J, 11, 53-62, 1997). We are not aware of any examples of Arabidopsis genes which respond to 6DOG or 3OMG yet this is an area of wide significance. Identification of such a gene would help to establish if a hexokinase-independent signalling system operates in plants, and would provide a basis for establishment of a genetic screen for mutants, using the gene promoter linked to a reporter such as luciferase. The aim of this proposal is to discover any genes which are either activated or repressed by glucose AND by 3OMG and/or 6DOG, but not by mannitol (an osmotic control). The use of both 3OMG and 6DOG will help to identify non-specific effects of either. All substrates will first be analysed by HPLC to confirm that they are pure. Arabidopsis Col-0 seedlings will be grown in vitro for 7 days in the absence of sugars, then treated with 30 mM glucose or glucose analogue for 8 h (these conditions are based on concentrations and time courses of Roitsch et al.). RNA will then be isolated from multiple independent plates to minimise biological variation.

Experimenter name = Dorthe Villadsen
Experimenter phone = 0131 650 5318
Experimenter fax = 0131 650 5392
Experimenter address = Institute of Cell and Molecular Biology
Experimenter address = University of Edinburgh
Experimenter address = The King_s Buildings
Experimenter address = Mayfield Road
Experimenter address = Edinburgh
Experimenter zip/postal_code = EH9 3JH
Experimenter country = UK
Keywords: growth_condition_design
 
Overall design 6 samples were used in this experiment
 
Contributor(s) Villadsen D, Smith S, Townsend H, Emmerson Z, Schildknecht B
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 01, 2006
Last update date Aug 28, 2018
Contact name Nottingham Arabidopsis Stock Centre (NASC)
E-mail(s) affy@arabidopsis.info
Phone +44 (0)115 951 3237
Fax +44 (0)115 951 3297
URL http://arabidopsis.info/
Organization name Nottingham Arabidopsis Stock Centre (NASC)
Department School of Biosciences, University of Nottingham
Street address Sutton Bonington Campus
City Loughborough
ZIP/Postal code LE12 5RD
Country United Kingdom
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM133711 Villadsen_A-1-villa-zer_SLD
GSM133712 Villadsen_A-2-villa-wat_SLD
GSM133713 Villadsen_A-3-villa-glc_SLD
Relations
BioProject PRJNA97021

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5723_RAW.tar 13.7 Mb (http)(custom) TAR (of CEL)

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