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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 24, 2014 |
| Title |
Altered mRNA expression profile of GW4064 treated mouse livers compared to vehicle control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Farnesoid X receptor (FXR) is a ligand activated nuclear receptor belonging to the nuclear receptor superfamily. Bile acids (BAs) are the endogenous ligand for FXR. FXR is a master regulator of BA homestasis, including BA synthesis, metabolism, transport, and enterohepatic circulation of BAs. Besides, FXR is involved in regulating diverse physioligical function in both humans and mice.
GW4064 is a synthetic FXR agonist which selectively activates FXR and induce the transcription of FXR target genes.
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| Overall design |
In this study, we treated wild type C57BL/6J mice with GW4064 at 100mg per kg body weight or vehicle control. Mice were treated three times, first dose at 8 am, second dose at 6 pm, third dose at 8 am the second day. Mice were sacrificed 2 hours after the last dose, and liver tissues were harvested for analysis. Animal protocols and procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Kansas Medical Center. Total RNA from livers was prepared with TRIzol Reagent (Invitrogen, CA), and the whole transcription expression levels were determined using Mouse Gene 1.0 ST Array system manufactured by Affymetrix, Inc..
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| Contributor(s) |
Guo GL, Kong B, Zhan L |
| Citation(s) |
25198545 |
| Submission date |
May 05, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
Le Zhan |
| E-mail(s) |
zhanle999@gmail.com
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| Phone |
9135530042
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| Organization name |
Rutgers University
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| Department |
Pharmacology and Toxicology
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| Lab |
EOHSI 332
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| Street address |
170 Frelinghuysen Rd
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| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE57312 |
ChIP-seq and RNA-seq study of human FXR in primary human hepatocytes and microarray analysis of GW4064 versus vehicle treated wild type mouse livers |
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| Relations |
| BioProject |
PRJNA246228 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57305_GW_vs_vehicle_WT_mice.Liver.txt.gz |
5.1 Kb |
(ftp)(http) |
TXT |
| GSE57305_RAW.tar |
12.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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