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| Status |
Public on Sep 01, 2006 |
| Title |
Expression data from ARPE-19 cells treated with LDL or ox-LDL |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
LDL or Ox-LDL 200ug/ml, which showed no loss of viability after a 48 hour exposure, induced a physiological and pathological transcriptional response, respectively. LDL induced a downregulation of genes associated with cholesterol biosynthesis while ox-LDL induced transcriptional alterations in genes related to inflammation, matrix expansion, lipid metabolism and processing, and apoptosis. Pentraxin-3 was secreted into the culture medium after RPE cells were stimulated with ox-LDL, and immunohistochemically evident in Bruch’s membrane of human macular samples with age-related macular degeneration. ARPE-19 cells exposed to 200?g/ml ox-LDL had a 38% apoptosis rate compared to less than 1% when exposed to LDL or untreated controls (p<0.0001).
While LDL induced a physiologic response by RPE cells, a pathological phenotypic response was seen after treatment with oxidatively modified LDL. The transcriptional, biochemical, and functional data provide initial support of a role for the hypothesis that modified LDLs are one trigger for initiating events that contribute to the development of age-related macular degeneration.
Keywords: treatment with non-treatment control
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| Overall design |
Human ARPE-19 cells were exposed to LDL or oxidatively modified LDL (ox-LDL) for 48 hours for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine whether retina, pigment epithelial cells develop a pathologic phenotype after exposure to low density lipoproteins (LDL) that are oxidatively modified.We have made two comparsions: LDL treatment versus non-treatment; ox-LDL treatment versus non-treatment.
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| Contributor(s) |
Yamada Y, Tian J, Yang Y, Cutler R, Wu T, Handa JT |
| Citation(s) |
18182060 |
| Submission date |
Sep 01, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yanqin Yang |
| Organization name |
The Johns Hopkins University
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| Street address |
600 N. Wolfe Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA97055 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5741_RAW.tar |
70.2 Mb |
(http)(custom) |
TAR (of CEL) |
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