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Series GSE57659 Query DataSets for GSE57659
Status Public on May 14, 2014
Title Transcription profiling by array of adipose samples from LDLR-/- mice to study response to anti-diabetic drug and dietary lifestyle interventions
Organism Mus musculus
Experiment type Expression profiling by array
Summary Lean male mice were fed a high fat diet (HFD, lard 24% w/w) for 16 weeks. At 9 weeks, when all hallmarks of prediabetes were established, groups of mice were treated with drug (rosiglitazone, pioglitazone, T0901317, or salicylate) for another 7 weeks together with the high fat diet. An additional group was switched back to a chow diet (dietary lifestyle intervention) after the first 9 weeks of high fat diet. All groups were compared to a control group receiving HFD alone and to a reference group fed chow (baseline reference) for the entire experimental period (16 weeks).
 
Overall design One group remained on maintenance chow throughout the entire study period (16 weeks) and served as healthy, age-matched control (n=9, samples hybridized: 7 for gonadal, 7 for subcutaneous, 8 for visceral). After the nine week run-in period, the HFD fed mice were matched into thirteen groups based on body weight. The first group was sacrificed immediately after matching (n=9, samples hybridized: 8 for gonadal, 8 for gonadal, 6 for visceral). The second group was continued on HFD until the end of the experiment at t=16 weeks (n=15, samples hybridized: 8 for gonadal, 7 for subcutaneous, 6 for visceral). The fourth group was switched to regular chow (dietary lifestyle intervention, n=9, samples hybridized: 8 for gonadal, 8 for subcutaneous, 7 for visceral). The other groups continued on HFD supplemented with drugs typically used in clinical practice. More specifically, following drugs were mixed into HFD: rosiglitazone (0.010% w/w, n=9, samples hybridized: 8 for all adipose depots), pioglitazone (0.010% w/w, n=9, samples hybridized: 8 for gonadal, 8 for subcutaneous, 6 for visceral), T0901317 (0.010% w/w, n=9, samples hybridized: 7 for all adipose depots) and salicylate (0.40% w/w, n=9, samples hybridized: 8 for all adipose depots).
 
Contributor(s) Radonjic M, Wielinga PY, Kelder T, Goelela VS, Verschuren L, van Duyvenvoorde W, Stroeve JH, Cnubben N, Kooistra T, van Ommen B, Kleemann R
Citation(s) 23457508
Submission date May 14, 2014
Last update date Jun 14, 2018
Contact name Thomas Kelder
Organization name TNO
Department Microbiology & Systems Bioloby
Street address Utrechtseweg 48
City Zeist
ZIP/Postal code 3704HE
Country Netherlands
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (179)
GSM1386042 16wkHFD_g_202
GSM1386043 16wkHFD_g_205
GSM1386044 16wkHFD_g_207
Relations
BioProject PRJNA247476

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57659_RAW.tar 3.1 Mb (http)(custom) TAR
GSE57659_non_normalized.txt.gz 28.2 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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