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| Status |
Public on Sep 01, 2014 |
| Title |
MMSET regulation of gene expression in t(4;14)+ myeloma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We investigated gene expression in isogenic myeloma cell lines with or without overexpressed MMSET protein. MMSET is a histone methyltransferase which methylates lysine 36 on histone H3. Overexpression of MMSET in myeloma leads to a global increase in H3K36 methylation and concomitant decrease in H3K27 methylation. These global changes in histone methylation lead to altered gene expression.
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| Overall design |
Total RNA was extracted from parental KMS11 cell line, NTKO cells which have the wild type MMSET allele inactivated and TKO cells which have overexpressed MMSET allele inactivated. Two different TKO clones were tested. Each sample was analyzed in triplicate.
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| Contributor(s) |
Popovic R |
| Citation(s) |
25188243 |
| Submission date |
May 21, 2014 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Relja Popovic |
| E-mail(s) |
r-popovic@northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
303 East Superior Street
|
| City |
Chicago |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA248290 |
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