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Series GSE57863 Query DataSets for GSE57863
Status Public on Sep 01, 2014
Title MMSET regulation of gene expression in t(4;14)+ myeloma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We investigated gene expression in isogenic myeloma cell lines with or without overexpressed MMSET protein. MMSET is a histone methyltransferase which methylates lysine 36 on histone H3. Overexpression of MMSET in myeloma leads to a global increase in H3K36 methylation and concomitant decrease in H3K27 methylation. These global changes in histone methylation lead to altered gene expression.
 
Overall design Total RNA was extracted from parental KMS11 cell line, NTKO cells which have the wild type MMSET allele inactivated and TKO cells which have overexpressed MMSET allele inactivated. Two different TKO clones were tested. Each sample was analyzed in triplicate.
 
Contributor(s) Popovic R
Citation(s) 25188243
Submission date May 21, 2014
Last update date Feb 18, 2019
Contact name Relja Popovic
E-mail(s) r-popovic@northwestern.edu
Organization name Northwestern University
Street address 303 East Superior Street
City Chicago
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL6884 Illumina HumanWG-6 v3.0 expression beadchip
Samples (12)
GSM1395235 WT1
GSM1395236 WT2
GSM1395237 WT3
Relations
BioProject PRJNA248290

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57863_RAW.tar 6.3 Mb (http)(custom) TAR
GSE57863_non-normalized.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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