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| Status |
Public on Apr 20, 2016 |
| Title |
Transcriptomics to discover splice variants involved in the browning of adipose tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Adipose tissue is the major depot for energy storage. Recent studies have shown that at least three types of adipocytes can be distinguished depending on their anatomical locations : 1) The classic brown adipocytes, i.e., brown adipose tissue (BAT); 2) The 'brite' (brown-in-white) adipocytes, i.e. inguinal white adipose tissue (iWAT); 3) The 'true' white adipocytes, i.e., epididymal white adipose tissue (eWAT). Two strains of mice (SV129 and C57BL/6J) were used in this study. SV strain is resistant to obesity and latter is prone to obesity. Pre-adipocyte cells were isolated from subcutaneous tissue (iWAT) to create four groups of cell cultures per strain of mouse.
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| Overall design |
Cells were cultured for either 3 days or 7 days. At 3 days the cells are still proliferating and are not considered mature adipocytes, whereas adipocytes at day 7 are considered as mature. One of the 7 days cell cultures was treated with norepinephrine (NE) and another 7 days cell culture was treated with rosiglitazone (R) for the entire experiment. The remaining two cell cultures (3 and 7 days) were remained untreated. One untreated cell culture was harvested after 3 days of growth, whilst the rest were harvested after 7 days of growth.
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| Contributor(s) |
Timmons J |
| Citation(s) |
27095197, 27488235 |
| Submission date |
May 22, 2014 |
| Last update date |
May 03, 2019 |
| Contact name |
James A Timmons |
| E-mail(s) |
jamie.timmons@gmail.com
|
| Organization name |
Augur Precision Medicine LTD
|
| Street address |
Cambridge
|
| City |
Cambridge |
| ZIP/Postal code |
CB1 |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA248389 |
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