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Series GSE57935 Query DataSets for GSE57935
Status Public on Jun 21, 2014
Title Expression data for Control and SMRT-Depleted MCF-7 Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Estrogens are an important regulator of breast cancer disease progression, and they function by binding the estrogen receptor-α (ERα) to regulate changes in gene expression. ERα is able to both activate and inhibit gene transcription in a gene-specific manner and do so by binding target DNA sequences and recruiting coactivators and corepressors which can modulate the chromatin environment. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) is known to act as coactivator and corepressor of ERα in a gene-specific manner.
We used a microarray analysis to examine the gene expression changes that occur when the coregulator SMRT is depleted from the ERα positive MCF-7 breast cancer cell line.
Overall design We sought to determine the genes that are regulated by depletion of the coregulator SMRT using Affymetrix Human Gene 1.0 ST Array. To this end, we transfected MCF-7 cells with control siRNA or SMRT-targeting siRNA for 48 h and treated for an additional 4 or 24 h with vehicle (0.1% EtOH) or 1 nM estradiol (E2). A total of 24 samples were analyzed, separated into eight groups each with three experimental replicates in each group, siControl-Veh 4 h, siControl -E2 4 h, siSMRT-Veh 4 h, siSMRT-E2 4 h, siControl-Veh 24 h, siControl-E2 24 h, siSMRT-Veh 24 h, siSMRT-E2 24 h.
Contributor(s) Karmakar S, Blackmore JK, Wang L, Li W, Smith CL
Citation(s) 24971610
Submission date May 23, 2014
Last update date Jul 26, 2018
Contact name Carolyn L Smith
Organization name Baylor College of Medicine
Department Molecular and Cellular Biology
Street address One Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (24)
GSM1397810 MCF-7_siCtrl_4h_Veh_rep1
GSM1397811 MCF-7_siCtrl_4h_Veh_rep2
GSM1397812 MCF-7_siCtrl_4h_Veh_rep3
BioProject PRJNA251372

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Supplementary file Size Download File type/resource
GSE57935_RAW.tar 135.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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