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| Status |
Public on Apr 22, 2016 |
| Title |
Expression data from HK2 proximal tubule cells transduced with shPGC-1alpha compared to cells transduced with control shRNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify regulation of genes involved in lipid and glycogen metabolism by PGC-1alpha
We used whole genome expression analysis to explore the mechanism of lipid and glycogen accumulation in proximal tubule cells following suppression of PGC-1alpha expression.
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| Overall design |
HK2 proximal tubule cell line were transduced with control (shGFP) or shPGC-1alpha shRNA. Successfully infected cells were selected with puromycin. Total cellular RNA was harvested 5 days after transduction, and 24 hours after puromycin selection had been ceased.
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| Contributor(s) |
Giaccia AJ, LaGory EL |
| Citation(s) |
26119730 |
| Submission date |
May 27, 2014 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Amato Giaccia |
| Organization name |
Stanford University
|
| Department |
Radiation Oncology
|
| Street address |
269 Campus Dr, CCSR South Rm 1250
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM1399306 |
HK2 shPGC-1alpha - biological replicate 1 |
| GSM1399307 |
HK2 shPGC-1alpha - biological replicate 2 |
| GSM1399308 |
HK2 shPGC-1alpha - biological replicate 3 |
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| Relations |
| BioProject |
PRJNA248663 |
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