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| Status |
Public on Jun 01, 2014 |
| Title |
Gene expression profiling of Wt1 mutant renal anlagen |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Wt1 is required for renal development and homozyogus knockout mice show renal agenesis caused by apoptosis of the metanepric mesnchyme.
To identify genes regulated by WT1, we performed comparative gene expression profiling on kidney rudmients of Wt1 heterozygous and homozygous mutant E10.25 embryos.
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| Overall design |
To identify deregulated genes, timed matings were performed and embryos isolated at E10.25, a time point before the onset of apoptosis in Wt1 mutants. The region containing the metanephric mesenchyme was microdissected, RNA isolated and after labeling, hybridized to Mouse Genome Survey Microarrays. Deregulated genes were identified using GeneSpring (GSX) software.
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| Contributor(s) |
Lecca R, Badro D, Brändli A, Schedl A |
| Citation(s) |
25031030 |
| Submission date |
May 29, 2014 |
| Last update date |
Sep 06, 2014 |
| Contact name |
Andreas Schedl |
| E-mail(s) |
schedl@unice.fr
|
| Organization name |
University of Nice
|
| Department |
iBV
|
| Street address |
Parc Valrose
|
| City |
Nice |
| ZIP/Postal code |
06100 |
| Country |
France |
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| Platforms (1) |
| GPL2995 |
ABI Mouse Genome Survey Microarray |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA249083 |
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