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Series GSE58129 Query DataSets for GSE58129
Status Public on Feb 08, 2017
Title Postnatal Development is Associated with Genome-scale Changes in DNA methylation in Mouse Liver
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation marks are thought to be set up during early development and to remain static thereafter in healthy tissues. Here, we characterize the liver DNA methylation patterns of mice before birth, during early postnatal development (from birth until weaning) and in adulthood. Our analyses show extensive epigenetic reprogramming in the liver occurring during postnatal development. 118,877 of 261,317 CpGs analyzed throughout the genome significantly changed their methylation level by more than 5% from birth to nine weeks of age, with some changing by up to 86%. Interestingly, changes in DNA methylation occur primarily in intergenic enhancer regions while gene promoters seem little affected. Analysis of 166 CpGs at multiple time points by locus-specific bisulfite sequencing reveals that this reprogramming primarily occurs between postnatal day 1 and day 20. This time period coincides with two major cellular changes in the liver: the differentiation of hepatocytes and extensive cell division. While cell multiplication leaves a distinct footprint on the DNA methylation patterns, we show that the extensive epigenetic reprogramming likely results from differentiation of hepatoblasts into hepatocytes. Overall, our data suggest that epigenetic remodeling is an important aspect of normal liver maturation and involves a large number of gene enhancers.
 
Overall design Methylation was measured across three ages by RRBS in 21 samples, and by locus specific bisulfite sequencing across eight ages in 31 samples, RRBS samples from another study (9wk old animals(GSM1262175, GSM1262176, GSM1262177, GSM1262187, GSM1262166, GSM1262167, GSM1262202)) were used to compare to P1 samples
 
Contributor(s) Cannon MV, Pilarowski G, Liu X, Serre DA
Citation(s) 27652892
Submission date May 30, 2014
Last update date May 15, 2019
Contact name Matt Cannon
E-mail(s) matthewvc1@gmail.com
Organization name Nationwide Children's Hospital
Department Center for Childhood Cancer
Street address 700 Children's Dr.
City Columbus
State/province OH
ZIP/Postal code 43205
Country USA
 
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (55)
GSM1401430 10-44-L-E18.5-M-Liver
GSM1401431 20-17-L-E18.5-M-Liver
GSM1401432 6-10-L-E18.5-F-Liver
Relations
BioProject PRJNA249080
SRA SRP042603

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58129_LocusSpecificSummary.txt.gz 74.0 Kb (ftp)(http) TXT
GSE58129_RrbsSummaryE18-P1.txt.gz 7.4 Mb (ftp)(http) TXT
GSE58129_RrbsSummaryP1-9wk.txt.gz 7.5 Mb (ftp)(http) TXT
GSE58129_mm10RawMethylationData.txt.gz 16.4 Mb (ftp)(http) TXT
GSE58129_mm9RawMethylationData.txt.gz 16.3 Mb (ftp)(http) TXT
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