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| Status |
Public on Jun 02, 2014 |
| Title |
M-CSF priming of osteoclast precursors can cause osteoclastogenesis-insensitivity, which can be prevented and overcome on bone |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Comparison of gene expression of the osteoclast precursor myeloid blast seeded on plastic and on bone, primed with M-CSF for 4 days and culture with M-CSF and RANKL for 1 day.
Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bone marrow precursor myeloid blast was pre-cultured with M-CSF on plastic and on bone. M-CSF priming prior to stimulation with M-CSF and osteoclast differentiation factor RANKL resulted in a complete loss of osteoclastogenic potential without bone. This coincided with a steeply decreased expression of osteoclast genes TRACP and DC-STAMP, but an increased expression of the macrophage markers F4/80 and CD11b. Compellingly, M-CSF priming on bone accelerated the osteoclastogenic potential: M-CSF primed cells that had received only one day M-CSF and RANKL and were grown on bone already expressed an array of genes that are associated with osteoclast differentiation and these cells differentiated into osteoclasts within 2 days. This implies that adhesion to bone dictates the fate of osteoclast precursors. Common macrophage-osteoclast precursors may become insensitive to differentiate into osteoclasts and regain osteoclastogenesis when bound to bone or when in the vicinity of bone.
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| Overall design |
Two conditions: Osteoclast precursors on plastic and on bone, n=4, dye swap
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| Contributor(s) |
de Vries TJ, Schoenmaker T, Aerts D, Grevers LC, Souza PP, Nazmi K, van de Wiel M, Ylstra B, van Lent PL, Leenen PJ, Everts V |
| Citation(s) |
24962140 |
| Submission date |
Jun 02, 2014 |
| Last update date |
May 10, 2018 |
| Contact name |
Teun J. de Vries |
| E-mail(s) |
teun.devries@acta.nl
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| Phone |
+31 20 5980223
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| Organization name |
Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University
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| Department |
Periodontology
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| Street address |
Gustav Mahlerlaan 3004
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| City |
Amsterdam |
| ZIP/Postal code |
1081 LA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (1) |
| GSM1402442 |
Primed for 4 days with M-CSF and 1 day M-CSF + RANKL |
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| Relations |
| BioProject |
PRJNA251382 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58146_RAW.tar |
68.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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