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Status |
Public on Jun 15, 2014 |
Title |
Oxidative Stress and Reduced Responsiveness of Challenged Circulating Leukocytes Following Pulmonary Instillation of Metal-Rich Particulate Matter in Rats |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Exposure to welding fume consists of a mixed exposure of metal-rich particulate matter with gases (ozone, carbon monoxide) and/or vapors (VOCs). Data suggests that welders are immune compromised. Given the inability of pulmonary leukocytes to properly respond to a secondary infection, the question arose concerning how far upstream the dysfunction persisted. Our aim was to evaluate circulating leukocyte functionality after welding fume exposure. The results showed that welding fume exposure has rapid effects on the circulating leukocyte population as identified by relative mRNA expression changes. Instillation of MMA-SS reduced inflammatory protein production of circulating leukocytes when challenged with the secondary stimulus LPS. The effects were not related to transcription, but were observed in conjunction with oxidative stress.
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Overall design |
Rats were intratracheally instilled (ITI) with phosphate buffered saline or 2 mg of welding fume collected from a manual metal arc-stainles steel weld. Rats were sacrificed 4 and 24 h post-exposure and whole blood was collected. Whole blood was used for cellular differential counts, RNA isolation with subsequent microarray and Ingenuity Pathway Analysis, and secondary stimulation with lipopolysaccharide utilizing TruCulture technology from Myriad/Rules Based Medicine. Also, mononuclear cells were isolated 24 h post-exposure to measure oxidative stress by flow cytometry and confocal microscopy. Whole blood collected 4 and 24 h post-exposure was challenged (a paired non-stimulant (null) and stimulant (LPS) tube) for 24 h using Truculture technology from Myriad/ RBM. After the challenge, cells were recovered using RNA later for subsequent RNA isolation and microarray.
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Contributor(s) |
Erdely A, Zeidler-Erdely PC |
Citation(s) |
25123171 |
Submission date |
Jun 03, 2014 |
Last update date |
Sep 15, 2014 |
Contact name |
Patti Christine Zeidler-Erdely |
E-mail(s) |
paz9@cdc.gov
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Phone |
3042855881
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Organization name |
CDC/NIOSH
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Department |
HELD
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Street address |
1095 Willowdale Road
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City |
Morgantown |
State/province |
WV |
ZIP/Postal code |
26505 |
Country |
USA |
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Platforms (1) |
GPL6101 |
Illumina ratRef-12 v1.0 expression beadchip |
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Samples (48)
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Relations |
BioProject |
PRJNA251553 |