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| Status |
Public on Oct 14, 2003 |
| Title |
Gene expression of transplanted hearts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
C57/BL6 to C57/BL6
Balb/c to C57/BL6
Balb/c to C57/BL6 with anti CD80/86 mAb
Identificaton of gene expression profile in tolerizing murine cardiac allograft by co-stimulatory blockade.
The induction of specific tolerance would be the ultimate achievement in transplant immunology, but the precise mechanisms of immunological tolerance remain largely unknown. Here, we investigated global gene expression analysis in tolerizing murine cardiac allografts by means of oligonucleotide microarrays. Tolerance induction was achieved in cardiac allografts from BALB/c to C57BL/6 mice by daily intraperitoneal injection of anti-CD80 and CD86 mAbs. Comparative analysis revealed 64 genes to be induced more extensively in the tolerizing than in the syngeneic isografts, and 16 genes than in the rejecting allografts. Two genes were specifically upregulated in the tolerizing allografts. In the tolerizing allografts there were induced marked expressions of a number of genes for proinflammatory factors, including interferon-gamma inducible cytokines and chemokines, as well as apoptosis-related genes which were also upregulated in the rejecting allografts. Moreover, these gene expression patterns continued to be upregulated more than 70 days post transplant. These results provide evidence that immunological tolerance can be induced and maintained in the presence of prominent proinflammatory gene expression in vivo.
Keywords: other
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| Contributor(s) |
Matsui Y, Saiura A, Sugawara Y, Sata M, Naruse K, Yagita H, Kohro T, Mataki C, Izumi A, Yamaguchi T, Minami T, Sakihama T, Ihara S, Aburatani H, Hamakubo T, Kodama T, Makuuchi M |
| Citation(s) |
12966135 |
| Submission date |
Aug 08, 2003 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Yuichi Matsui |
| E-mail(s) |
ymatsui-tky@umin.ac.jp
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| Phone |
81-3-5452-5230
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| Organization name |
University of Tokyo
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| Department |
Biology
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| Lab |
Laboratory for System Biology and Medicine
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| Street address |
Komaba 4-6-1
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| City |
Meguro-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (14)
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| GSM8929 |
Tolerizing allografts 7 days No.3 |
| GSM8930 |
Tolerizing allografts 70 days No.3 |
| GSM8931 |
Isograft 7 days No.1 |
| GSM8932 |
Isograft 7 days No.2 |
| GSM8933 |
Isograft 70 days No.1 |
| GSM8934 |
Rejecting allografts 7 days No.1 |
| GSM8935 |
Rejecting allografts 7 days No.2 |
| GSM8936 |
Tolerizing allografts 7 days No.1 |
| GSM8937 |
Tolerizing allografts 7 days No.2 |
| GSM8938 |
Tolerizing allografts 70 days No.1 |
| GSM8939 |
Tolerizing allografts 70 days No.2 |
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| Relations |
| BioProject |
PRJNA85365 |
| Supplementary data files not provided |
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