|
| Status |
Public on Nov 01, 2014 |
| Title |
Gene expression profiling of mouse bone marrow-derived macrophages (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Bone marrow (BM) cells were obtained by flushing the long bones of 8-week old C57BL/6 mice. BM cells were then were plated in macrophage SFM medium (Life Technologies) supplemented with penicillin-streptomycin and CSF-1 (Peprotech, 100 ng/ml) and cultured for one week to allow macrophage differentiation. BMDMs were polarized by adding IL-4 to the medium (40 ng/ml, Peprotech) for 72h or left untreated.
|
| |
|
| Overall design |
8 samples total, 2 groups, 4 biological replicates in each group
|
| |
|
| Contributor(s) |
Squadrito ML, Baer C, Burdet F, Maderna C, Gilfillan GD, Lyle R, Ibberson M, De Palma M |
| Citation(s) |
25159140 |
| Submission date |
Jun 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Mark Ibberson |
| Organization name |
SIB Swiss Institute of Bioinformatics
|
| Department |
Vital-IT
|
| Street address |
Genopode building
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA251892 |
| SRA |
SRP043011 |
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