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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 17, 2014 |
| Title |
Gene expression profiling of recent thymic emigrants (RTEs) from a mouse strain with an ENU-induced mutation in Zfp335 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The generation of naïve T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Mutant blt/blt mice exhibit a naïve T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. Zfp335 binds to promoter regions via a consensus motif, and its target genes are enriched in categories related to protein metabolism, mitochondrial function and transcriptional regulation. Restoring the expression of one target, Ankle2, partially rescues T cell maturation. Our findings identify Zfp335 as a transcription factor and essential regulator of late-stage intrathymic and post-thymic T cell maturation.
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| Overall design |
WT and blt/blt CD4+ RTEs were sorted from mixed hematopoietic chimeras for RNA extraction and gene expression analysis on Affymetrix arrays. 6 samples were analyzed in total, with 3 biological replicates per genotype.
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| Contributor(s) |
Han BY, Wu S, Foo C, Horton RM, Jenne CN, Watson SR, Whittle B, Goodnow CC, Cyster JG |
| Citation(s) |
25343476, 27401554 |
| Submission date |
Jun 06, 2014 |
| Last update date |
Aug 10, 2016 |
| Contact name |
Brenda Yuyuan Han |
| Organization name |
Institute of Molecular and Cell Biology, A*STAR
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| Street address |
61 Biopolis Drive, Proteos
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| City |
Singapore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
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| Platforms (1) |
| GPL17777 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: mogene10st_Mm_ENTREZG_17.1.0] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE58293 |
Zinc finger protein Zfp335 is required for formation of the naïve T cell compartment |
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| Relations |
| BioProject |
PRJNA251900 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58289_RAW.tar |
50.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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