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Series GSE58303 Query DataSets for GSE58303
Status Public on Jun 10, 2014
Title Mouse brain tissue after methanol and normal saline treatment
Organism Mus musculus
Experiment type Expression profiling by array
Summary We recently showed that methanol emitted by wounded plants might function as a signaling molecule for plant-to-plant and plant-to-animal communications. In mammals, methanol is considered a poison because the enzyme alcohol dehydrogenase (ADH) converts methanol into toxic formaldehyde. However, the detection of methanol in the blood and exhaled air of healthy volunteers suggests that methanol may be a chemical with specific functions rather than a metabolic waste product. Using a genome-wide analysis of the mouse brain, we demonstrated that an increase in blood methanol concentration led to a change in the accumulation of mRNAs from genes primarily involved in detoxification processes and regulation of the alcohol/aldehyde dehydrogenases gene cluster. Removal of the intestine significantly decreased the rate of methanol addition to the plasma and suggested that the gut flora may be involved in the endogenous production of methanol. Liver mRNA quantification showed changes in the accumulation of mRNAs from genes involved in cell signalling and detoxification processes. We hypothesized that endogenous methanol acts as a regulator of homeostasis by controlling the mRNA synthesis
 
Overall design 4 mouse were treated with methanol for 2 h and 4 with normal saline for 2 h, than we collected brain tissue samples and extracted total RNA by Trizol according to manufacturer's protocol
 
Contributor(s) Shindyapina AV, Petrunia IV
Citation(s) 25033451
Submission date Jun 09, 2014
Last update date Jun 14, 2018
Contact name Anastasia Shindyapina
E-mail(s) shindyapina.a@gmail.com
Organization name MSU Lomonosov
Department Biengineering
Street address Mosfilmovskaya 76
City Moscow
State/province Moscow
ZIP/Postal code 119192
Country Russia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1406243 methanol-treated 1
GSM1406244 methanol-treated 2
GSM1406245 methanol-treated 3
Relations
BioProject PRJNA251961

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58303_RAW.tar 3.1 Mb (http)(custom) TAR
GSE58303_non-normalized.txt.gz 882.7 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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