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| Status |
Public on Jun 10, 2014 |
| Title |
Gene expression profiling in MCF-7 cells exposed to blackcurrant extract. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Blackcurrants (Ribes nigrum L., Grossulariaceae) have a high content of anthocyanin polyphenols and these have been shown to have beneficial effects on health, owing to their antioxidant and anti-carcinogenic properties. This study analyzed the constituents of blackcurrant extract (BCE) and investigated its potential phytoestrogenic effects using a breast cancer cell line (MCF-7) overexpressing the estrogen receptor (ER) α. Microarray and ingenuity pathway analysis showed that BCE activated upstream genes such as ERα and transforming growth factor beta 1, and upregulated the expression of many genes downstream of ERα.
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| Overall design |
MCF-7 cells were seeded in culture dish and maintain to confluent. Then medium replace with phenol-red-serum-free DMEM medium with or without BCE (50μg/ml). After the cells were incubated for 24 h at 37 °C 5% CO2.
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| Citation(s) |
26395027 |
| Submission date |
Jun 09, 2014 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Naoki Nanashima |
| E-mail(s) |
n_nanashima@auhw.ac.jp
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| Organization name |
Aomori University of Health and Welfare
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| Department |
Department of Nutrition
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| Street address |
58-1 Mase, Hamadate
|
| City |
Aomori |
| State/province |
Aomori |
| ZIP/Postal code |
030-8505 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA251962 |
