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Series GSE58315 Query DataSets for GSE58315
Status Public on Nov 12, 2014
Title Transcriptome Analysis of the Human Corneal Endothelium
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Defining the normal and age-dependent HCEnC transcriptome will further refine our understanding of the functional roles that the endothelium plays in the cornea and will provide a basis upon which to compare transcriptomes of normal and dystrophic endothelium for the subsequent development of gene-targeted therapies.
We used microarrays to comprehensively characterize human corneal endothelial cell (HCEnC) gene expression, age-dependent differential gene expression and to identify expressed genes mapped to chromosomal loci associated with the corneal endothelial dystrophies PPCD1, FECD4 and XECD
 
Overall design Eleven corneas from six pediatric (4, 6, 10,11, 17 and 18 years old) and five adult (53, 56, 57, 64 and 70 years old) donors, were obtained from various eye banks . Descemet and corneal endothelium were removed from donor eye bank corneas as intact sheets utilizing a technique that we have adapted for ex vivo donor tissue. Total RNA was isolated from the corneal endothelium using TriReagent and subsequently purified with the RNeasy Clean-Up Kit. The integrity of the isolated RNA was analyzed using the Agilent 2100 Electrophoresis Bioanalyzer System and found to be of sufficient quantity (approximately 1ug) and quality (RNA integrity number in the range of 8.3 to 9.0) for analysis using the Affymetrix 1.1ST gene chip arrays.
 
Contributor(s) Frausto RF, Wang C, Aldave AJ
Citation(s) 25377225
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 EY022082 Identification and Characterization of the Genetic Basis of PPCD UNIVERSITY OF CALIFORNIA LOS ANGELES ALDAVE
Submission date Jun 09, 2014
Last update date Nov 08, 2016
Contact name Anthony J. Aldave
E-mail(s) Aldave@jsei.ucla.edu
Organization name Stein Eye Institute, UCLA
Department Ophthalmology
Lab Cornea Genetics Laboratory
Street address 200 Stein Plaza DSERC 3-143
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (11)
GSM1406377 HCEnC, pediatric, preadolescent 1
GSM1406378 HCEnC, adult, adult 2
GSM1406379 HCEnC, pediatric, preadolescent 3
Relations
BioProject PRJNA251984

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Supplementary file Size Download File type/resource
GSE58315_RAW.tar 45.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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