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| Status |
Public on Dec 31, 2015 |
| Title |
Long non-coding Satellite III RNAs link the heat shock response and cell proliferation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Genomes of complex organisms encode a wide variety of long non-coding RNAs (lncRNAs) that regulate gene expression. In human cells exposed to heat stress, transcription of lncRNAs is highly induced from Satellite III sequences located primarily within locus 9q12. Expression of Sat III transcripts is dependent on heat shock factor 1, the master regulator of the heat shock response. Sat III transcripts serve as the backbone of specific subnuclear structures called nuclear Stress Bodies, nSBs. Despite being known for two decades, the function of nSBs and Sat III transcripts is unknown, which prompted us to conduct RNA profiling in human cells where Sat III transcripts were either intact or downregulated. Comparison of these cells revealed that under stress conditions, Sat III transcripts are involved in regulating a plethora of genes, including such associated with cell growth and protein homeostasis. Our results show that the presence of Sat III transcripts has a repressive effect on cell proliferation. We propose a model in which the inducible expression of Sat III transcripts restrains cell proliferation, allowing the cell to assess whether to grow or undergo apoptosis, and thereby suggesting a function for these lncRNAs in cell fate decisions and in restoring protein homeostasis.
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| Overall design |
Total RNA from HEK293T cells transfected with siRNA against Satellite III transcripts or scrambled siRNA and heat shocked 1 h at 42°C , or heat shocked 1 h at 42 °C followed by recovery for 6 h, or left untreated was compared.
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| Contributor(s) |
Sandqvist A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 12, 2014 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Lea Sistonen |
| Organization name |
Åbo Akademi
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| Department |
Department of Biosciences
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| Street address |
Tykistökatu 6 B
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| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (22)
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| GSM1411103 |
scrambled transfection, replicate 1 |
| GSM1411104 |
scrambled transfection, heat shock treatment, replicate 1 |
| GSM1411105 |
scrambled transfection, heat shock treatment followed by recovery, replicate 1 |
| GSM1411106 |
siRNA against Sat III transfection, replicate 1 |
| GSM1411107 |
siRNA against Sat III transfection, heat shock treatment, replicate 1 |
| GSM1411108 |
siRNA against Sat III transfection, heat shock treatment followed by recovery, replicate 1 |
| GSM1411109 |
scrambled transfection, replicate 2 |
| GSM1411110 |
scrambled transfection, heat shock treatment, replicate 2 |
| GSM1411111 |
scrambled transfection, heat shock treatment followed by recovery, replicate 2 |
| GSM1411112 |
siRNA against Sat III transfection, replicate 2 |
| GSM1411113 |
siRNA against Sat III transfection, heat shock treatment, replicate 2 |
| GSM1411114 |
siRNA against Sat III transfection, heat shock treatment followed by recovery, replicate 2 |
| GSM1411115 |
scrambled transfection, replicate 3 |
| GSM1411116 |
scrambled transfection, heat shock treatment, replicate 3 |
| GSM1411117 |
scrambled transfection, heat shock treatment followed by recovery, replicate 3 |
| GSM1411118 |
siRNA against Sat III transfection, heat shock treatment followed by recovery, replicate 3 |
| GSM1411119 |
scrambled transfection, replicate 4 |
| GSM1411120 |
scrambled transfection, heat shock treatment, replicate 4 |
| GSM1411121 |
scrambled transfection, heat shock treatment followed by recovery, replicate 4 |
| GSM1411122 |
siRNA against Sat III transfection, replicate 4 |
| GSM1411123 |
siRNA against Sat III transfection, heat shock treatment, replicate 4 |
| GSM1411124 |
siRNA against Sat III transfection, heat shock treatment followed by recovery, replicate 4 |
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| Relations |
| BioProject |
PRJNA252616 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58439_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR |
| GSE58439_non-normalized.txt.gz |
6.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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