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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 08, 2014 |
| Title |
CaM Kinase II mediates maladaptive post-infarct remodeling but not acute myocardial ischemia/reperfusion injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Calcium/calmodulin-dependent protein kinase II (CaMKII) was suggested to mediate ischemic myocardial injury and adverse cardiac remodeling. However, the specific functions of the CaMKII isoforms and splice variants in ischemia/reperfusion (I/R) injury have not been investigated yet. Thus, we studied the roles of the CaMKII isoforms and splice variants in I/R by the use of various CaMKII mutant mice. CaMKIIδC was up-regulated already one day after I/R injury but surprisingly, acute I/R injury was neither affected in CaMKIIδ-deficient mice, CaMKIIδ-deficient mice in which the splice variants CaMKIIδB and C were re-expressed nor in conditional CaMKIIδ/γ double-knockout mice (DKO). In contrast, 5 weeks after I/R, DKO mice were protected against extensive scar formation and cardiac dysfunction. Leukocyte infiltration was not altered one day but five days after I/R, explaining the late effects of CaMKII deletion on post-I/R remodeling. Other than reported before, we demonstrate that CaMKII is not critically involved in the immediate mechanisms that regulate acute I/R injury but in the process of post-infarct remodeling.
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| Overall design |
We analysed 6 groups in total: 3 groups from wild-type control animals and 3 groups from CaMKII delta/gamma double-KO mice. Sham operated animals served as controls in both wild-type and KO animal groups. 2 different time points in ischia/reperfusion operated animals were investigated: 1 day and 5 days post-surgery.
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| Contributor(s) |
Weinreuter M, Kreusser MM, Beckendorf J, Schreiter FC, Leuschner F, Lehmann LH, Hofmann KP, Diemert N, Rostosky J, Volz HC, Jungmann A, Nickel A, Sticht C, Kretz N, Maack C, Schneider MD, Gröne HJ, Müller OJ, Katus HA, Olson EN, Backs J |
| Citation(s) |
25193973 |
| Submission date |
Jun 13, 2014 |
| Last update date |
Oct 05, 2017 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
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| Department |
ZMF
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| Street address |
Theodor-Kutzer-Ufer
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| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
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| Platforms (1) |
| GPL18802 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [mogene20st_Mm_ENTREZG_18.0.0] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA252736 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58486_RAW.tar |
181.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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