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Series GSE5861 Query DataSets for GSE5861
Status Public on Sep 19, 2006
Title Searching for Brca1 regulated X-linked genes
Organism Mus musculus
Experiment type Expression profiling by array
Summary We compared the expression levels of X-linked genes in the mammary glands of Brca1 D11/D11;p53+/- mutant and control (p53+/-) mice at three different stages of the mammary cycle: virgin, pregnant day 16.5, and lactation day 1, using a cDNA microarray. In about 690 X-linked genes that are expressed at these three stages of mammary cycle of development, we found 16 X-linked genes showed altered expression levels in Brca1 D11/D11;p53+/- mammary glands in comparison with controls at all three time points. Among them, 9 genes were up-regulated and 7 were down-regulated. This result indicates that mutation of Brca1 could affect expression of a few X-linked genes in mammary tissues. However, this was unlikely caused by failure of X chromosome inactivation, as seven of them were down-regulated, and Xist RNA was expressed in all the Brca1 mutant mammary tissues.

Keywords: BRCA1 mutation analysis
Overall design Mutations in breast cancer associated gene-1 (BRCA1) are associated with hereditary breast and ovarian cancers. BRCA1 encodes a tumor suppressor protein that functions in DNA repair, transcriptional regulation, chromatin remodeling, centrosome duplication and checkpoint control, etc.. We were interested in fishing out the genes regulated by Brca1. Three pairs of mammary glands from Brca1 D11/D11;p53+/- mutant and control (p53+/-) mice at three different stages of the mammary cycle were analyzed by microarray for gene expression levels.
Contributor(s) Xiao C, Xu X, Wang R, Li W, Deng C
Citation(s) 17350580
Submission date Sep 18, 2006
Last update date Feb 11, 2019
Contact name Cuiying Xiao
Organization name NIH
Department NIDDK
Street address 10 Center Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM126600 02-07-05_Deng_Mouse430_2_796
GSM126601 02-07-05_Deng_Mouse430_2_801
GSM126602 02-07-05_Deng_Mouse430_2_I442
BioProject PRJNA97269

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