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Series GSE58656 Query DataSets for GSE58656
Status Public on Mar 31, 2016
Title A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity (BeadChip)
Organism Mus musculus
Experiment type Expression profiling by array
Summary We address the molecular mechanisms through which Myc supports mESC identity, by performing gene expression profile analyses of Myc- and LIF-maintained ESCs, together with Epiblast Stem Cell (EpiSC), as a control for a primed state of stemness. We then investigated whether the Myc-driven self-reinforcing circuit, installed in Myc-dependent ESCs, could support the maintenance of ES cell identity in absence of the originating stimulus, namely the MycER activation achieved by OHT stimulation. To this end, we derived ESCs from Myc-dependent cells, following MycER inactivation upon withdrawal of OHT and profile their gene expression.

 
Overall design Total RNA extracted from LIF-, Myc- and -Myc-ESCs at the indicated passages were processed to profile gene expression by microarray analysis. Gene-expression profiling was also perfomed on EpiSC as control for primed stem cell state.
 
Contributor(s) Fagnocchi L, Hatsuda H, Zippo A
Citation(s) 27301576
Submission date Jun 19, 2014
Last update date Jun 14, 2018
Contact name Alessio Zippo
E-mail(s) zippo@ingm.org
Organization name National Institute of Molecular Genetics
Street address Via Francesco Sforza, 35
City Milan
ZIP/Postal code 20122
Country Italy
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (19)
GSM1416500 R1 Myc-ER mESC LIF rep.1
GSM1416501 R1 Myc-ER mESC LIF rep.2
GSM1416502 R1 Myc-ER mESC LIF rep.3
This SubSeries is part of SuperSeries:
GSE80558 A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity
Relations
BioProject PRJNA253113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58656_RAW.tar 3.1 Mb (http)(custom) TAR
GSE58656_non-normalized.txt.gz 2.1 Mb (ftp)(http) TXT
GSE58656_non-normalized_2.txt.gz 1.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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