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Status |
Public on Aug 01, 2014 |
Title |
Time course and dose response analysis of gene expression in BCR or CD40-stimulated DT40 B cell |
Organism |
Gallus gallus |
Experiment type |
Expression profiling by array
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Summary |
Gene Expression dynamics is important information. To know IKK- or ERK-dependent B cell receptor- or CD40-induced gene expression dynamics, we performed the time course and dose response analysis in wild type or MEK inhibitor treated or IKKbeta inactive DT40 B cells.
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Overall design |
Two replicated samples were analysed. Unstimulated cells (T0) were control. WT cells or MEK Inh. (MEK inhibitor;U0126 5mM, 30min pretreated) or IKKbetaSA (S176/181A knock-in) Cells were stimulated with 0.1, 1, 10 µg/ml of anti-IgM (M4) or 1, 3, 6 µg/ml of CD40ligand (CD40L) for 0, 15, 30, 45, 60 or 90min.
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Contributor(s) |
Shinohara H |
Citation missing |
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Submission date |
Jul 01, 2014 |
Last update date |
Aug 02, 2014 |
Contact name |
Hisaaki Shinohara |
E-mail(s) |
qshino@rcai.riken.jp
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Organization name |
RIKEN IMS
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Lab |
Laboratory for Integrated Cellular Systems
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Street address |
1-7-22 Suehiro-cho
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City |
Yokohama |
ZIP/Postal code |
230-0045 |
Country |
Japan |
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Platforms (1) |
GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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Samples (53)
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GSM1423730 |
WT,T15m,M4:0.1 |
GSM1423731 |
WT,T30m,M4:0.1 |
GSM1423732 |
WT,T45m,M4:0.1 |
GSM1423733 |
WT,T60m,M4:0.1 |
GSM1423734 |
WT,T90m,M4:0.1 |
GSM1423735 |
WT,T15m,M4:1 |
GSM1423736 |
WT,T30m,M4:1 |
GSM1423737 |
WT,T45m,M4:1 |
GSM1423738 |
WT,T60m,M4:1 |
GSM1423739 |
WT,T90m,M4:1 |
GSM1423740 |
WT,T15m,M4:10 |
GSM1423741 |
WT,T30m,M4:10 |
GSM1423742 |
WT,T45m,M4:10 |
GSM1423743 |
WT,T60m,M4:10 |
GSM1423744 |
WT,T90m,M4:10 |
GSM1423745 |
IKKbetaSA,T15m,M4:10 |
GSM1423746 |
IKKbetaSA,T30m,M4:10 |
GSM1423747 |
IKKbetaSA,T45m,M4:10 |
GSM1423748 |
IKKbetaSA,T60m,M4:10 |
GSM1423749 |
IKKbetaSA,T90m,M4:10 |
GSM1423750 |
MEK Inh.,T15m,M4:10 |
GSM1423751 |
MEK Inh.,T30m,M4:10 |
GSM1423752 |
MEK Inh.,T45m,M4:10 |
GSM1423753 |
MEK Inh.,T60m,M4:10 |
GSM1423754 |
MEK Inh.,T90m,M4:10 |
GSM1423755 |
WT,T15m,CD40L:1 |
GSM1423756 |
WT,T30m,CD40L:1 |
GSM1423757 |
WT,T45m,CD40L:1 |
GSM1423758 |
WT,T60m,CD40L:1 |
GSM1423759 |
WT,T90m,CD40L:1 |
GSM1423760 |
WT,T15m,CD40L:3 |
GSM1423761 |
WT,T30m,CD40L:3 |
GSM1423762 |
WT,T45m,CD40L:3 |
GSM1423763 |
WT,T60m,CD40L:3 |
GSM1423764 |
WT,T90m,CD40L:3 |
GSM1423765 |
WT,T15m,CD40L:6 |
GSM1423766 |
WT,T30m,CD40L:6 |
GSM1423767 |
WT,T45m,CD40L:6 |
GSM1423768 |
WT,T60m,CD40L:6 |
GSM1423769 |
WT,T90m,CD40L:6 |
GSM1423770 |
MEK Inh.,T15m,CD40L:6 |
GSM1423771 |
MEK Inh.,T30m,CD40L:6 |
GSM1423772 |
MEK Inh.,T45m,CD40L:6 |
GSM1423773 |
MEK Inh.,T60m,CD40L:6 |
GSM1423774 |
MEK Inh.,T90m,CD40L:6 |
GSM1423775 |
IKKbetaSA,T15m,CD40L:6 |
GSM1423776 |
IKKbetaSA,T30m,CD40L:6 |
GSM1423777 |
IKKbetaSA,T45m,CD40L:6 |
GSM1423778 |
IKKbetaSA,T60m,CD40L:6 |
GSM1423779 |
IKKbetaSA,T90m,CD40L:6 |
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Relations |
BioProject |
PRJNA254076 |
Supplementary file |
Size |
Download |
File type/resource |
GSE59002_RAW.tar |
501.7 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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