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Series GSE5921 Query DataSets for GSE5921
Status Public on Sep 26, 2007
Title Differential expression of BMP4-regulated genes associated with commitment of C3H10T1/2 cells into adipocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary C3H10T1/2 stem cells are committed to the adipocyte lineage by treatment with BMP-4 and grown to postconfluence. When subjected to our standard differentiation protocol, the committed cells differentiate into adipocytes in a manner indistinguishable from that of 3T3-L1 preadipocytes. In contrast, C3H10T1/2 cells not committed with BMP-4 remain undifferentiated despite treatment with differentiation inducers. The molecular basis of the commitment process, however, has not been elucidated. Since postconfluent uncommitted and committed C3H10T1/2 cells respond differently to the differentiation inducers, it was reasoned that the two cell types differed at the gene expression level. Therefore, we undertook microarray gene expression profiling to detect changes between the two cell populations at postconfluence to identify expressed genes that may be responsible for the dramatic change in phenotype.
Keywords: control vs treated
Overall design C3H10T1/2 cells were plated and treated with 50 ng/ml BMP4 and grown to postconfluence. Control samples were treated in the same manner were not given BMP4. Total RNA was collected at postconfluence. There were 3 replicates each for control and treated cells.
Contributor(s) Otto TC, Bowers RR, Lane D
Citation(s) 17204246
Submission date Sep 26, 2006
Last update date Feb 11, 2019
Contact name M. Daniel Lane
Organization name Johns Hopkins University
Street address 725 N. Wolfe St
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM137318 10T1/2 cells_treated_BMP4_rep1
GSM137325 10T1/2 cells_treated_BMP4_rep2
GSM137326 10T1/2 cells_treated_BMP4_rep3
BioProject PRJNA97365

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Supplementary file Size Download File type/resource
GSE5921_RAW.tar 37.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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