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Series GSE59347 Query DataSets for GSE59347
Status Public on Jul 12, 2014
Title mRNA expression profile in IL-4-treated macrophages transfected with miR-142-5p ASO or miR-130a-3p mimics
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify target genes of miR-142-5p and miR-130a-3p that are involved in M2 polarization, we examined the mRNA expression profile changes after altering miR-142-5p or miR-130a-3p expression in IL-4-treated macrophages.
Overall design Macrophages were transfected with control ASO, miR-142-5p ASO, control mimics or miR-130-3p mimics using lentiviral vectors.Each treatment contained 3 replicates. After 24 hr, the cells were treated with IL-4 for 24h.mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified, transcribed into fluorescent cRNA along the entire length of the transcripts without 3' bias utilizing a random priming method and labeled by Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242). The labeled cRNAs were hybridized onto the Agilent_Whole Human Genome Microarray 4x44K v2. After having washed the slides, the arrays were scanned by the Agilent Microarray Scanner (Agilent p/n G2565BA).
Contributor(s) Su S
Citation(s) 26436920
Submission date Jul 11, 2014
Last update date Jun 07, 2019
Contact name Shicheng Su
Phone 84-02-87332022
Organization name Sun-Yat-Sen Memorial Hospital
Street address 107 Yanjiang West Road
City Guangzhou
ZIP/Postal code 510120
Country China
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (12)
GSM1694101 IL-4-treated macrophages transfected with miR-142-5p ASO-1
GSM1694102 IL-4-treated macrophages transfected with miR-142-5p ASO-2
GSM1694103 IL-4-treated macrophages transfected with miR-142-5p ASO-3
This SubSeries is part of SuperSeries:
GSE59349 The differential miRNA expression induces M2 macrophage polarization and mediates their profibrogenic effects
BioProject PRJNA255114

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Supplementary file Size Download File type/resource
GSE59347_RAW.tar 23.4 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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