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Series GSE59347 Query DataSets for GSE59347
Status Public on Jul 12, 2014
Title mRNA expression profile in IL-4-treated macrophages transfected with miR-142-5p ASO or miR-130a-3p mimics
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify target genes of miR-142-5p and miR-130a-3p that are involved in M2 polarization, we examined the mRNA expression profile changes after altering miR-142-5p or miR-130a-3p expression in IL-4-treated macrophages.
 
Overall design Macrophages were transfected with control ASO, miR-142-5p ASO, control mimics or miR-130-3p mimics using lentiviral vectors.Each treatment contained 3 replicates. After 24 hr, the cells were treated with IL-4 for 24h.mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified, transcribed into fluorescent cRNA along the entire length of the transcripts without 3' bias utilizing a random priming method and labeled by Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242). The labeled cRNAs were hybridized onto the Agilent_Whole Human Genome Microarray 4x44K v2. After having washed the slides, the arrays were scanned by the Agilent Microarray Scanner (Agilent p/n G2565BA).
 
Contributor(s) Su S
Citation(s) 26436920
Submission date Jul 11, 2014
Last update date Jun 07, 2019
Contact name Shicheng Su
E-mail(s) seasonso@163.com
Phone 84-02-87332022
Organization name Sun-Yat-Sen Memorial Hospital
Street address 107 Yanjiang West Road
City Guangzhou
ZIP/Postal code 510120
Country China
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (12)
GSM1694101 IL-4-treated macrophages transfected with miR-142-5p ASO-1
GSM1694102 IL-4-treated macrophages transfected with miR-142-5p ASO-2
GSM1694103 IL-4-treated macrophages transfected with miR-142-5p ASO-3
This SubSeries is part of SuperSeries:
GSE59349 The differential miRNA expression induces M2 macrophage polarization and mediates their profibrogenic effects
Relations
BioProject PRJNA255114

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59347_RAW.tar 23.4 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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