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Series GSE59348 Query DataSets for GSE59348
Status Public on Jul 12, 2014
Title M2 cytokines alter the miRNA expression profile in macrophages
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To examine the miRNA expression profile of the profibrogenic macrophages, we compared miRNA expression in untreated versus IL-4 or IL-13-treated macrophages using miRNA microarray.IL-4 and IL-13 treatment resulted in marked changes in 18 and 19 miRNAs in macrophages, respectively.(n=3, fold changes>2, P<0.05)
Overall design Total RNA from human primary macrophages (n=3) treated with or without 20 ng/ml IL-4 or 20ng/ml IL-13 for 48 hr was isolated with a mirVana miRNA Isolation kit (Ambion, Foster, CA). The miRNA expression profile of the samples were examined using miRCURY LNA™ arrays (v18.0; covering 1921 human miRNAs, Exiqon ,Vedbaek, Denmark).
Contributor(s) Su S
Citation(s) 26436920
Submission date Jul 11, 2014
Last update date Jun 07, 2019
Contact name Shicheng Su
Phone 84-02-87332022
Organization name Sun-Yat-Sen Memorial Hospital
Street address 107 Yanjiang West Road
City Guangzhou
ZIP/Postal code 510120
Country China
Platforms (1)
GPL17107 Exiqon miRCURY LNA™ microRNA Array - hsa, mmu & rno
Samples (9)
GSM1435486 control macrophages 1
GSM1435487 IL-4 treated macrophages 1
GSM1435488 IL-13 treated macrophages 1
This SubSeries is part of SuperSeries:
GSE59349 The differential miRNA expression induces M2 macrophage polarization and mediates their profibrogenic effects
BioProject PRJNA255113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59348_Normalized_data.txt.gz 108.3 Kb (ftp)(http) TXT
GSE59348_RAW.tar 370.0 Kb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data are available on Series record

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