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Series GSE5940 Query DataSets for GSE5940
Status Public on Sep 30, 2006
Title Gene Expression Profiling of Primary Rat Oligodendrocytes
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Oligodendrocytes undergo extensive changes as they differentiate from progenitors into myelinating cells. To better understand the
molecular mechanisms underlying this transformation, we performed a comparative analysis using gene expression profiling of A2B5+
oligodendrocyte progenitors and O4+ oligodendrocytes. Cells were sort-purified ex vivo from postnatal rat brain using flow cytometry.
Using Affymetrix microarrays, 1707 transcripts were identified with a more than twofold increase in expression inO4+oligodendrocytes.
Many genes required for oligodendrocyte differentiation were upregulated in O4+ oligodendrocytes, including numerous genes encoding
myelin proteins. Transcriptional changes included genes required for cell adhesion, actin cytoskeleton regulation, and fatty acid and
cholesterol biosynthesis. At the O4+ stage, there was an increase in expression of a novel proline-rich transmembrane protein (Prmp).
Localized to the plasma membrane, Prmp displays adhesive properties that may be important for linking the extracellular matrix to the
actin cytoskeleton. Together, our results highlight the usefulness of this discovery-driven experimental strategy to identify genes relevant
to oligodendrocyte differentiation and myelination.
Keywords: develepmental stage, oligodendrocyte differentiation
 
Overall design Whole brain dissociates were prepared from one litter of 10 male postnatal day 7 rat pups for each of the 5 A2B5 bioligcal replicates and the 4 O4+ bioligical replicates. Total RNA was extracted from single A2B5+ and single O4+ cells sorted directly from postnatal day7 rat whole brain dissociates using flow cytometry.
 
Contributor(s) Nielsen JA, Maric D, Lau P, Barker JL, Hudson LD
Citation(s) 17005852
Submission date Sep 28, 2006
Last update date Mar 03, 2017
Contact name Joe A Nielsen
E-mail(s) nielsenj@ninds.nih.gov
Phone 301-402-7350
Organization name NIH/NINDS
Lab Section of Develpmental Genetics
Street address 49 Convent Drive BD49,5A75
City Bethesda
State/province MD
ZIP/Postal code 20892-4479
Country USA
 
Platforms (2)
GPL341 [RAE230A] Affymetrix Rat Expression 230A Array
GPL342 [RAE230B] Affymetrix Rat Expression 230B Array
Samples (18)
GSM138218 A2B5-1
GSM138219 A2B5-2
GSM138220 A2B5-3
Relations
BioProject PRJNA97387

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