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| Status |
Public on May 26, 2015 |
| Title |
Genome-wide methylation profiles of forensically relevant body fluids [Set1] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
The ability to predict tissue type and donor’s age from molecular profiles of crime scene samples has practical implications in forensics. In order to identify body fluid- and age-associated DNA methylation changes, genome-wide DNA methylation profiling was carried out for body fluids including blood, saliva, semen, menstrual blood, and vaginal fluid obtained from individuals aged 20 to 59. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs in bisulfite converted DNA. Samples included 12 of each blood, saliva and semen samples from 18 male donors aged 20 to 59, and 3 of each vaginal fluid and menstrual blood samples from 4 female donors in their twenties.
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| Overall design |
Genome-wide DNA methylation profiling of body fluids obtained from young and old individuals. The Illumina Infinium 450K Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 450K CpGs from human body fluids including blood, saliva, semen, vaginal fluid and menstrual blood.
Bisulfite converted DNA from the 24 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip
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| Contributor(s) |
Lee HY |
| Citation(s) |
25796047 |
| Submission date |
Jul 17, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Hwan Young Lee |
| E-mail(s) |
hylee192@snu.ac.kr
|
| Phone |
+82-2-740-8352
|
| Organization name |
Seoul National University College of Medicine
|
| Department |
Forensic Medicine
|
| Street address |
103 Daehak-ro, Jongno-gu
|
| City |
Seoul |
| ZIP/Postal code |
03080 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (24)
|
| GSM1438499 |
genomic DNA from saliva of male 50M01 |
| GSM1438500 |
genomic DNA from saliva of male 50M05 |
| GSM1438501 |
genomic DNA from saliva of male 50M07 |
| GSM1438502 |
genomic DNA from semen of male 20M02 |
| GSM1438503 |
genomic DNA from semen of male 20M03 |
| GSM1438504 |
genomic DNA from semen of male 20M06 |
| GSM1438505 |
genomic DNA from semen of male 50M04 |
| GSM1438506 |
genomic DNA from semen of male 50M05 |
| GSM1438507 |
genomic DNA from semen of male 50M06 |
| GSM1438508 |
genomic DNA from vaginal swab of female 20F02 |
| GSM1438509 |
genomic DNA from vaginal swab of female 20F06 |
| GSM1438510 |
genomic DNA from vaginal swab of female 20F07 |
| GSM1438511 |
genomic DNA from menstrual blood of female 20F02 |
| GSM1438512 |
genomic DNA from menstrual blood of female 20F06 |
| GSM1438513 |
genomic DNA from menstrual blood of female 20F09 |
| GSM1438514 |
genomic DNA from whole blood of male 20M01 |
| GSM1438515 |
genomic DNA from whole blood of male 20M05 |
| GSM1438516 |
genomic DNA from whole blood of male 20M06 |
| GSM1438517 |
genomic DNA from whole blood of male 50M01 |
| GSM1438518 |
genomic DNA from whole blood of male 50M04 |
| GSM1438519 |
genomic DNA from whole blood of male 50M06 |
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| This SubSeries is part of SuperSeries: |
| GSE59509 |
Genome-wide methylation profiles of forensically relevant body fluids |
|
| Relations |
| BioProject |
PRJNA255471 |
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