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Series GSE5959 Query DataSets for GSE5959
Status Public on Oct 29, 2006
Title Expression differences in the liver of a congenic mouse with low serum IGF-1
Organism Mus musculus
Experiment type Expression profiling by array
Summary Several studies have shown that bone mineral density (BMD), a clinically measurable predictor of osteoporotic fracture, is the sum of genetic and environmental influences. In addition, serum IGF-1 levels have been correlated to both BMD and fracture risk. We previously identified a Quantitative Trait Locus (QTL) for Bone Mineral Density (BMD) on mouse Chromosome (Chr) 6 that overlaps a QTL for serum IGF-1. The B6.C3H-6T (6T) congenic mouse is homozygous for C57BL/6J (B6) alleles across the genome except for a 30 cM region on Chr 6 that is homozygous for C3H/HeJ (C3H) alleles. This mouse was created to study biology behind both the BMD and the serum IGF-1 QTLs and to identify the gene(s) underlying these QTLs. Female 6T mice have lower BMD and lower serum IGF-1 levels at all ages measured. As the liver is the major source of serum IGF-1, we examined differential expression in the livers of fasted female B6 and 6T mice by microarray.
Keywords: Genetic variation
Overall design The experimental design of this experiment was a simple two-factor experiment, with three biological replicates of each factor (in this case mouse strain, B6.C3H-6T vs. C57BL/6J). Each sample represented one mouse; no samples were pooled. No technical replicated were done.
Contributor(s) Ackert-Bicknell CL, Churchill GA, Affourtit J, Beamer WG, Rosen CJ
Citation(s) 17584978
Submission date Oct 03, 2006
Last update date Feb 11, 2019
Contact name Cheryl Lynne Ackert-Bicknell
Organization name The Jackson Laboratory
Street address 600 Main Street
City Bar Harbor
State/province ME
ZIP/Postal code 04609
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM138289 Liver_Chow_C57BL/6J_Female_8wks_Ped#36
GSM138290 Liver_Chow_C57BL/6J_Female_8wks_Ped#37
GSM138291 Liver_Chow_C57BL/6J_Female_8wks_Ped#38
BioProject PRJNA97423

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5959_RAW.tar 37.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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