|
| Status |
Public on Dec 30, 2016 |
| Title |
mRNA expression in human DAOY cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generate ZNF423 knockdown and control DAOY cells with lentivirus that co-expressed the fluorescent protein mCherry. We performed whole genome RNA sequencing (RNA-seq) of three batched of cultured ZNF423 KD or control KD cells. The sequence reads were analyzed by Homer followed by edgeR. The analyzed RNA-seq results showed differential expression profile including 12 known cilia genes, and 3 of these were validated with qRT-PCR on mouse granule cell precursors. This study proved data how ZNF423 linked to cilia complexes.
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| |
|
| Overall design |
RNA-seq in three batched of control and ZNF423 KD cells(generated by lentivirus delivered shRNA targeting ZNF423 sequence).
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| |
|
| Contributor(s) |
Hamilton BA, Hong C |
| Citation(s) |
27727273 |
| Submission date |
Jul 18, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Bruce Hamilton |
| E-mail(s) |
bah@ucsd.edu
|
| Phone |
8588222298
|
| Organization name |
UC San Diego
|
| Department |
Medicine
|
| Lab |
CMM
|
| Street address |
9500 Gilman Dr.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA255674 |
| SRA |
SRP044651 |
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