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Series GSE59675 Query DataSets for GSE59675
Status Public on Jan 30, 2015
Title Expression analysis of superior cervical ganglion from backcrossed TH-MYCN transgenic mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Neuroblastoma susceptibility in TH-MYN mice is heavily influence by mouse strain background. We have combined linkage analysis of tumor susceptibility with expression QTL analysis of superior cervical ganglia (pure peripheral symptathetic nervous tissue) to identify genes underlying tumor formation
The development of targeted therapeutics for neuroblastoma, the third most common tumor in children, has been limited by a poor understanding of growth signaling mechanisms unique to the peripheral nerve precursors from which tumors arise. In this study, we combined genetics with gene expression analysis in the peripheral sympathetic nervous system to implicate arginase 1 and GABA signaling in tumor formation in vivo. In human neuroblastoma cells, activation of GABA-A receptors by a benzodiazepine induced apoptosis and inhibited mitogenic signaling through AKT and MAPK. These results suggest that GABA influences both neural development and neuroblastoma, and that benzodiazepines in clinical use may have potential for neuroblastoma therapy.
 
Overall design 224 backcrossed mice were genotyped at 349 markers to identify susceptibility loci for neuroblastoma. Gene expression from Superior Cervical Ganglia from 116 of these mice were correlated with genotyping data to identify putative eQTL.
 
Contributor(s) Hackett CS, Chen J, Quigley DA, Balmain A, Weiss WA
Citation(s) 25437558
Submission date Jul 22, 2014
Last update date Mar 06, 2018
Contact name William A. Weiss
Organization name University of California, San Francisco
Department Neurology
Lab William A. Weiss
Street address 1450 3rd Street, HD-220
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL6096 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]
Samples (116)
GSM1442362 backcross mouse ID 14446
GSM1442363 backcross mouse ID 14939
GSM1442364 backcross mouse ID 14978
Relations
BioProject PRJNA255915

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59675_RAW.tar 2.5 Gb (http)(custom) TAR (of CEL)
GSE59675_genotyping_data.txt.gz 16.5 Kb (ftp)(http) TXT
Processed data included within Sample table

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