NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE59766 Query DataSets for GSE59766
Status Public on Jul 26, 2014
Title Leishmania major Friedlin V1 lpg1- and lpg2- mutants differentially modulate the Interleukin-12 immune response in human dendritic cells.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Monocyte derived dendritic cells (MDDC) were infected with Leishmania major parasites which were knockout mutants of either lpg1 or lpg2 genes that responsible for expression of surface molecular structures on the parasites in order to assess the effects those molecules have on human host cell gene expression.
 
Overall design Monocyte derived dendritic cells (MDDC) were generated from blood buffy coats collected from four anonymous healthy human donors and infected 10:1 (parasite to host cell) with Leishmania major Friedlin V1 strain wildtype parasites and lipophosphogylcan mutants, where after total RNA was harvested, cDNA generated, and hybridized to human gene transcipt expression arrays to assess differential host cell gene transcriptional expression differences relative to uninfected cells.

Please note that the final RMA normalized background value for each array was provided in the characteristics field and any data that was below the background value was filtered out.
 
Contributor(s) Favila MA, Geraci NS, Hickerson S, Jayakumar A, Tripathi V, Turco SJ, Beverley SM, McDowell MA
Citation(s) 26630499
Submission date Jul 25, 2014
Last update date Dec 16, 2015
Contact name Mary Ann McDowell
E-mail(s) mcdowell.11@nd.edu
Organization name University of Notre Dame
Department Biological Sciences
Street address 210 Galvin Life Science Center
City Notre Dame
State/province Indiana
ZIP/Postal code 46556
Country USA
 
Platforms (1)
GPL16025 NimbleGen Homo sapiens Expression Array [100718_HG18_opt_expr]
Samples (24)
GSM1446219 RNA_human-monocyte-derived-dendritic-cell_Leishmania-major-strain-FV1-infected_8hpi_donor-170
GSM1446220 RNA_human-monocyte-derived-dendritic-cell_Leishmania-major-strain-FV1-infected_8hpi_donor-664
GSM1446221 RNA_human-monocyte-derived-dendritic-cell_Leishmania-major-strain-FV1-infected_8hpi_donor-665
Relations
BioProject PRJNA256176

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59766_RAW.tar 80.5 Mb (http)(custom) TAR (of PAIR)
Raw data provided as supplementary file
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap