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| Status |
Public on Apr 12, 2016 |
| Title |
A Novel Role of Polycomb in Positive Transcription Regulation by Modulating H4K20me1 |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Polycomb group (PcG) proteins were originally shown to play central roles in maintaining heritable gene suppression during the normal development of higher eukaryotes. However, recent studies in Drosophila and mammals imply a potential positive role of PcG proteins in gene transcription with unknown mechanism. Here, for the first time we report Pc-mediated, E(z)/H3K27me3-dependent positive transcription regulation of Ci and Sens, two key transcription factors involved in body plan during normal development. Further mechanistic studies showed that Pc regulates Sens expression by regulating H4K20me1 at Sens locus. More importantly, this mechanism applies to other genes involved in the developmental process. In summary, we report a novel and general transcription regulation mechanism, that is, Pc-mediated, E(z)/H3K27me3-dependent positive transcription regulation by modulating H4K20me1 during development.
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| Overall design |
H4K20me1 ChIP-seq dataset was generated in wide type Drosophila third instar larvae wing discs. RNA-seq datasets were generated for wide type, Polycomb RNAi samples.
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| Contributor(s) |
Lv X, Han Z, Chen H, Yang X, Zhang Z, Yang B, Xia Y, Lu Y, Pan C, Fu L, Han H, Mao F, Wu M, Zhou Z, Li D, Zhang L, Jiang H, Li L, Wei G, Zhao Y |
| Citation(s) |
27002220 |
| Submission date |
Jul 30, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Gang Wei |
| E-mail(s) |
weigang@picb.ac.cn
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| Organization name |
CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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| Lab |
Key Laboratory of Computational Biology
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| Street address |
320 Yue Yang Road
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA257070 |
| SRA |
SRP045094 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59924_RAW.tar |
12.9 Mb |
(http)(custom) |
TAR (of BED, BW) |
| GSE59924_all.genes.expression.in.FPKM.020215.txt.gz |
191.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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