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Series GSE59964 Query DataSets for GSE59964
Status Public on Aug 01, 2014
Title Gene expression profile of the Cmah gene depletion on mouse liver, lung, kidney and heart
Organism Mus musculus
Experiment type Expression profiling by array
Summary N-glycolylneuraminic acid (Neu5Gc) is generated by hydroxylation of CMP-Neu5Ac to CMP-Neu5Gc, catalyzed by CMP-Neu5Ac hydroxylase (CMAH). However, humans lack this common mammalian cell surface molecule, Neu5Gc, due to inactivation of the CMAH gene during evolution. CMAH is one of several human-specific genes whose function has been lost by disruption or deletion of the coding frame. It has been suggested that CMAH inactivation has resulted in biochemical or physiological characteristics that have resulted in human-specific diseases. To identify differential gene expression profiles associated with the loss of Neu5Gc expression, we performed microarray analysis using Illumina MouseRef-8 v2 Expression BeadChip, using the main tissues (liver, lung, kidney, and heart) from a control mouse and a Cmah-null mouse.
 
Overall design Total RNA was extracted and purified from the liver, lung, kidney, and heart of WT and Cmah-null mice using RNeasy columns (Qiagen; Valencia, CA, USA) according to the manufacturer’s protocol. The RNA quality was verified using an Agilent Bioanalyzer 2100 (Agilent Technologies; Palo Alto, CA, USA) using the RNA 6000 Pico Assay. Generation of double-stranded cDNA, preparation and labeling of cRNA, hybridization to Mouse Ref-8 v2.0 Expression BeadChip (Illumina, Inc.; San Diego, CA, USA), washing, and scanning were all performed according to the standard Illumina protocol. Arrays were scanned using the Illumina Bead Array Reader Confocal Scanner.
 
Contributor(s) Kwon D, Kim J
Citation(s) 25229777, 26558285
Submission date Jul 31, 2014
Last update date Jul 31, 2019
Contact name Deug-Nam Kwon
E-mail(s) sun-76@daum.net
Phone +82-2-450-3687
Organization name Konkuk University
Street address 1 hwayang-dong, Kwangjin-gu
City Seoul
ZIP/Postal code 143-701
Country South Korea
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM1462810 Wild type_lung_1
GSM1462811 Wild type_lung_2
GSM1462812 Wild type_lung_3
Relations
BioProject PRJNA257187

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59964_Non-normalized_data.txt.gz 7.1 Mb (ftp)(http) TXT
GSE59964_Normalized_data2_Heart.xlsx 24.7 Mb (ftp)(http) XLSX
GSE59964_Normalized_data2_Kidney.xlsx 27.8 Mb (ftp)(http) XLSX
GSE59964_Normalized_data2_Liver.xlsx 24.2 Mb (ftp)(http) XLSX
GSE59964_Normalized_data2_Lung.xlsx 29.3 Mb (ftp)(http) XLSX
GSE59964_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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