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| Status |
Public on Oct 11, 2006 |
| Title |
Gene Expression and Functional Evidence of Epithelial-to-Mesenchymal Transition in Papillary Thyroid Cancer Invasion |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Papillary thyroid cancers (PTC) that invade into local structures are associated with a poor prognosis, but the mechanisms for PTC invasion are incompletely defined limiting the development of new therapies. To characterize biological processes involved in PTC invasion, we analyzed the gene expression profiles of microscopically dissected intratumoral samples from central and invasive regions of seven widely invasive PTCs and normal thyroid tissue by oligonucleotide microarray and performed confirmatory expression and functional studies. In comparison to the central regions of primary PTCs, the invasive fronts overexpressed TGFbeta, NFkappaB and integrin pathway members, and regulators of small G-proteins and CDC42. Moreover, reduced levels of mRNAs encoding proteins involved in cell-cell adhesion and communication were identified, consistent with epithelial-to-mesenchymal transition (EMT). To confirm that aggressive PTCs were characterized by EMT, 35 additional PTCs were examined for expression of vimentin, a hallmark of EMT. Overexpression of vimentin was associated with PTC invasion and nodal metastasis. Functional, in vitro studies demonstrated that vimentin was required for the development and maintenance of both a mesenchymal morphology and invasiveness in thyroid cancer cells. We conclude that EMT is a common mechanism of PTC invasion and that vimentin regulates thyroid cancer EMT in vitro.
Keywords: Genetic modification
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| Overall design |
Total RNA was obtained from all 7 central and invasion regions, as well as from 4 of 7 normal tissues. In addition, the comparison of central vs. normal tissues were compared to 9 paired central and normal samples from The Ohio State University tumor bank simultaneously analyzed using the same methods
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| Contributor(s) |
Vasko V, Espinosa AV, Scouten W, He H, Auer H, Liyanarachchi S, Larin A, Savchenko V, Francis GL, de la Chapelle A, Saji M, Ringel MD |
| Citation(s) |
17296934 |
| Submission date |
Oct 10, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Sandya Liyanarachchi |
| Organization name |
OSU
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| Department |
Human Genetics
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| Lab |
de la Chapelle Lab
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| Street address |
Rm 830, 460, W 12th Avenue
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (18)
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| GSM139002 |
PC10: Normal thyroid paired with PC5 and PC6 |
| GSM139003 |
PC41: Normal thyroid paired with PC39 and PC40 |
| GSM139004 |
PC47: Normal thyroid paired with PC44 and PC45 |
| GSM139005 |
PC81: Normal thyroid paired with PC79 and PC80 |
| GSM139006 |
PC5: Thyroid cancer center area paired with PC10 and PC6 |
| GSM139007 |
PC39: Thyroid cancer center area paired with PC41 and PC40 |
| GSM139008 |
PC44: Thyroid cancer center area paired with PC47 and PC45 |
| GSM139009 |
PC79: Thyroid cancer center area paired with PC81 and PC79 |
| GSM139010 |
PC66: Thyroid cancer center area paired with PC67 |
| GSM139011 |
PC72: Thyroid cancer center area paired with PC73 |
| GSM139012 |
PC89: Thyroid cancer center area paired with PC90 |
| GSM139013 |
PC6: Thyroid cancer invasive area paired with PC10 and PC5 |
| GSM139014 |
PC40: Thyroid cancer invasive area paired with PC41 and PC39 |
| GSM139015 |
PC45: Thyroid cancer invasive area paired with PC47 and PC44 |
| GSM139016 |
PC80: Thyroid cancer invasive area paired with PC81 and PC79 |
| GSM139017 |
PC67: Thyroid cancer invasive area paired with PC66 |
| GSM139018 |
PC73: Thyroid cancer invasive area paired with PC72 |
| GSM139019 |
PC90: Thyroid cancer invasive area paired with PC89 |
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| Relations |
| BioProject |
PRJNA97795 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6004_RAW.tar |
144.2 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
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