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| Status |
Public on Sep 30, 2014 |
| Title |
Microrray expression data of Osteoarthritis synovial fibroblasts (OASF) transfected with TBX5 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
TBX5 is hypomethylated in Rheumatoid Arthritis synovial fibroblasts (RASF). Hypomethylation increased the TBX5 expression in RASF.
We performed a microarray expression analysis upon TBX5 overexpression to find TBX5 molecular targets.TBX5 belongs to the T-box transcription factor family all of which possess a T-box DNA binding domain in their sequence. It has been shown to play an important role in tissue development and cancer.
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| Overall design |
We were interested in identifying a possible role of TBX5 overexpression in RA. To that end, OASF were transfected with pcDNA3.1(+)_TBX5 or pcDNA3.1(+) empty vector as a negative control. After 48h, RNA was extracted and cDNA was generated by reverse transcription. The labeled cDNA was hybridized to the Affymetrix microarray.
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| Contributor(s) |
Karouzakis E, Neidhart M |
| Citation(s) |
25320281 |
| Submission date |
Aug 06, 2014 |
| Last update date |
Dec 02, 2014 |
| Contact name |
Emmanuel Karouzakis |
| E-mail(s) |
emmanuel.karouzakis@usz.ch
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| Organization name |
University Hospital Zurich
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| Department |
Center of Experimental Rheumatology
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| Street address |
Wagistrasse 14/3.OG
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| City |
Schlieren |
| ZIP/Postal code |
CH-8952 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA257652 |
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