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Series GSE6025

Query DataSets for GSE6025

Status

Public on May 01, 2007

Title

eif3h/WT transcript level

Organism

Arabidopsis thaliana

Experiment type

Expression profiling by array

Summary

Microarray comparisons of transcript level in wild-type Arabidopsis and eif3h mutant plants.
Goal:
To detect any change in transcript level between WT and eif3h mutant.
BACKGROUND: The eukaryotic translation initiation factor eIF3 has multiple roles during the initiation of translation of cytoplasmic mRNAs. However, the contributions of individual subunits of eIF3 to the translation of specific mRNAs remain poorly understood. RESULTS: Working with stable reporter transgenes in Arabidopsis thaliana it was demonstrated that the h subunit of
eIF3 contributes to the efficient translation initiation of mRNAs harboring upstream open reading frames (uORFs) in their 5’ leader sequence. uORFs, which can function as devices for translational regulation, are present in over 30% of Arabidopsis mRNAs, and are enriched among mRNAs for transcriptional regulators and protein modifying enzymes. Microarray comparisons of polysome loading in wild-type and eif3h mutant plants revealed that eIF3h generally helps to maintain efficient polysome loading of mRNAs harboring multiple uORFs. Independently, eIF3h also boosted polysome loading of mRNAs with long coding sequences. Moreover, the lesion in eIF3h revealed a concerted upregulation of translation for specific functional subgroups of mRNAs, including ribosomal proteins and proteins involved in photosynthesis.
CONCLUSIONS: The intact eIF3h protein contributes to efficient translation initiation on 5’ leader sequences harboring multiple uORFs, although mRNA features independent of uORFs were also implicated. Moreover, our data suggest that regulons of translational control can be revealed by mutations in generic translation initiation factors.
Keywords: mutant, total RNA

Overall design

Total RNA samples were isolated from 10-day-old wild-type and eif3h mutant plants

Contributor(s)

Kim B, von Arnim AG

Citation(s)

17439654

Submission date

Oct 12, 2006

Last update date

Aug 28, 2018

Contact name

Albrecht G von Arnim