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| Status |
Public on Nov 01, 2014 |
| Title |
Impact of HP1gamma and HP1gamma S83 phosphorylation on the transcriptionnal effect of PMA stimulation of mouse fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have here followed the transcriptional effect of stimulation with the phorbol ester PMA in mouse fibroblasts from HP1gamma null mice recomplemented with either wild-type HP1gamma or an HP1g with an S83A mutation
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| Overall design |
Spontaneously immortalized mouse embryonic fibroblasts from HP1gamma null mice were used to stably integrate either an empty expression vector, or expression vectors for either WT or S83A mutant HP1gamma. These cells were then stimulated with PMA for 0 or 60 min. and used for transcriptome analysis by Next Generation sequencing.
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| Contributor(s) |
ARBIBE L, RACHEZ C, CAMMAS F, MUCHARDT C |
| Citation(s) |
25216677 |
| Submission date |
Aug 11, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Christian MUCHARDT |
| E-mail(s) |
muchardt@pasteur.fr
|
| Phone |
0033145688525
|
| Organization name |
Institut Pasteur Paris
|
| Department |
Developmental and Stem Cell Biology
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| Lab |
Epigenetic Regulation
|
| Street address |
25 rue du Dr-Roux
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| City |
Paris cedex 15 |
| ZIP/Postal code |
75724 |
| Country |
France |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA259825 |
| SRA |
SRP045890 |
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