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Series GSE60303 Query DataSets for GSE60303
Status Public on Sep 08, 2014
Title Genome-wide analysis of stress-exposure-associated and exposure-related individual differences associated hippocampus gene expression in males and females.
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Delineating the molecular basis of individual differences in the stress response is critical to understanding the pathophysiology and treatment of posttraumatic stress disorder (PTSD). In this study, 7 d after predator-scent-stress (PSS) exposure, male and female rats were classified into vulnerable (i.e., “PTSD-like”) and resilient (i.e.,minimally affected) phenotypes on the basis of their performance on a variety of behavioral measures. Genome-wide expression profiling in blood and two limbic brain regions (amygdala and hippocampus), followed by quantitative PCR validation, was performed in these two groups of animals, as well as in an unexposed control group. Differentially expressed genes were identified in blood and brain associated with PSS-exposure and with distinct behavioral profiles postexposure. There was a small but significant between-tissue overlap (4–21%) for the genes associated with exposure-related individual differences, indicating convergent gene expression in both sexes. To uncover convergent signaling pathways across tissue and sex, upstream activated/deactivated transcription factorswere first predicted for each tissue and then the respective pathways were identified. Glucocorticoid receptor (GR) signaling was the only convergent pathway associatedwith individual differences when using the most stringent statistical threshold.
 
Overall design Total RNA obtained from hippocampus of rats exposed to predator-scent-stress and showing extreme (n=5 males and n=5 females) or minimal (n=5 males and n=5 females) behavioral response compared to unexposed to stress and behaviorally tested controls (n=5 males and n=5 females).
 
Contributor(s) Daskalakis NP, Cohen H, Cai G, Buxbaum JD, Yehuda R
Citation(s) 25114262
Submission date Aug 11, 2014
Last update date Sep 08, 2014
Contact name Nikolaos Daskalakis
E-mail(s) ndaskalakis@mclean.harvard.edu
Phone 6178552785
Organization name McLean Hospital/ Harvard Medical School
Department Department of Psychiatry
Lab Neurogenomics & Translational Bioinformatics Laboratory
Street address Oaks Building 328, Mailstop 212, 115 Mill Street
City Belmont
State/province MA
ZIP/Postal code 02478
Country USA
 
Platforms (1)
GPL6101 Illumina ratRef-12 v1.0 expression beadchip
Samples (30)
GSM1470661 Hippocampus_rat Control_rat F1
GSM1470662 Hippocampus_rat Control_rat F2
GSM1470663 Hippocampus_rat Control_rat F3
This SubSeries is part of SuperSeries:
GSE60304 PTSD model
Relations
BioProject PRJNA257991

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60303_RAW.tar 2.8 Mb (http)(custom) TAR
GSE60303_non-normalized.txt.gz 4.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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