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Series GSE6055 Query DataSets for GSE6055
Status Public on Dec 14, 2006
Title Gene Expression Profiling Reveals Unique Pathways Associated with Differential Severity of Lyme Arthritis
Organism Mus musculus
Experiment type Expression profiling by array
Summary The murine model of Lyme disease provides a unique opportunity to study the localized host response to similar stimulus, B. burgdorferi, in the joints of mice destined to develop severe arthritis (C3H) or mild disease (C57BL/6). Pathways associated with the response to infection and the development of Lyme arthritis were identified by global gene expression patterns using oligonucleotide microarrays. A robust induction of IFN responsive genes was observed in severely arthritic C3H mice at one week of infection, which was absent from mildly arthritic C57BL/6 mice. In contrast, infected C57BL/6 mice displayed a novel expression profile characterized by genes involved in epidermal differentiation and wound repair, which were decreased in the joints of C3H mice. These expression patterns were associated with disease state rather than inherent differences between C3H and C57BL/6 mice, as C57BL/6-IL10-/- mice infected with B. burgdorferi develop more severe arthritis that C57BL/6 mice and displayed an early gene expression profile similar to C3H mice. Gene expression profiles at two and four weeks post infection revealed a common response of all strains that was likely to be important for the host defense to B. burgdorferi and mediated by NF-kB-dependent signaling. The gene expression profiles identified in this study add to the current understanding of the host response to B. burgdorferi and identify two novel pathways that may be involved in regulating the severity of Lyme arthritis.
Keywords: disease state analysis, time course
 
Overall design Expression profiling of ankle tissues of C3H, C57BL/6, and C57BL/6-IL10-/- mice infected with B. burgdorfer (0, 1, 2, and 4 weeks post-infection)
 
Contributor(s) Crandall H, Dunn DM, Ma Y, Wooten RM, Zachary JF, Weis JH, Weis RB, Weis JJ
Citation(s) 17114465
Submission date Oct 17, 2006
Last update date Feb 11, 2019
Contact name Janis Weis
E-mail(s) janis.weis@path.utah.edu
Phone 801-581-8386
Fax 801-581-4517
Organization name University of Utah
Department Pathology
Street address 15 North Medical Drive East
City Salt Lake City
State/province UT
ZIP/Postal code 84112-5650
Country USA
 
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM140228 Expression data from ankle tissue of C3H mice at 0 week of infection (uninfected)
GSM140229 Expression data from ankle tissue of C3H mice at 1 week of infection
GSM140230 Expression data from ankle tissue of C3H mice at 2 week of infection
Relations
BioProject PRJNA97733

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6055_RAW.tar 59.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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