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Series GSE60654 Query DataSets for GSE60654
Status Public on Aug 27, 2014
Title Expression data from the bladders of carcinogen-treated mice with genetic Hh pathway manipulation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Attenuation of Hedgehog (Hh) pathway activity leads to accelerated tumor progression in a mouse model of N-butyl-N-4-hydroxybutyl nitrosamine (BBN) – induced bladder carcinoma.
In order to identify genes regulated by the Hh pathway that might be involved in bladder cancer progression, we performed transcriptional profiling of bladders harvested from mice after BBN-exposure, comparing Gli1CreER/WT; Smoflox/WT mice to Gli1CreER/WT; Smoflox/flox mice, which express CreER under control of the Gli1 promoter and carry one or two floxed alleles of the essential Hh pathway transductory component Smoothened (Smo) respectively. Administration of Tamoxifen to these mice results in attenuation of Hh pathway activty to a greater extent in the Gli1CreER/WT;Smoflox/flox mice as compared to Gli1CreER/WT;Smoflox/WT mice, allowing identification of Hh-pathway regulated genes.
 
Overall design 6 total samples were analyzed. 3 bladders from Gli1CreER/WT; Smoflox/WT mice and 3 bladders from Gli1CreER/WT; Smoflox/flox mice were analyzed.
 
Contributor(s) Lim A, Beachy PA
Citation(s) 25314078
Submission date Aug 22, 2014
Last update date Dec 05, 2014
Contact name Agnes Lim
Organization name Stanford University
Department Developmental Biology
Lab Philip Beachy
Street address 265 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL19108 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [MoEx10stv1_Mm_REFSEQ.cdf]
Samples (6)
GSM1484227 Gli1CreER/WT; Smoflox/WT BBN-treated bladder, biological rep 1
GSM1484228 Gli1CreER/WT; Smoflox/WT BBN-treated bladder, biological rep 2
GSM1484229 Gli1CreER/WT; Smoflox/WT BBN-treated bladder, biological rep 3
Relations
BioProject PRJNA259200

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60654_RAW.tar 198.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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