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| Status |
Public on Feb 04, 2015 |
| Title |
Expression data from Ezh2 epithelial knock-out mouse lungs at E14.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Ezh2 epigenetically suppresses developmentally-regulated genes. Ezh2 is highly expressed during development, including in the lung. We knocked out Ezh2 in the developing lung epithelium using a Shh-cre driver which is active in foregut endoderm prior to lung morphogenesis. Many developmentally regulated genes became derepressed in the mutant lungs, leading to defects in lung development.
Microarray analysis of genes upregulated in Ezh2 epithelial knock-out mouse lungs compared to Shh-cre controls.
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| Overall design |
We generated Ezh2 epithelial mutant lungs by crossing the Ezh2-floxed line (Su et al., 2003) with the Shh-cre line (Harfe et al., 2004). We isolated RNA from whole mouse lungs at embryonic day 14.5 from 4 Shh-cre:Ezh2-flox/flox embryos (M1-M4) and 4 Shh-cre embryos (C1-C4). Littermates are as follows: C1+C2; C3+C4; M1+M2; M3+M4.
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| Contributor(s) |
Snitow ME, Morley MP, Rathi KS, Morrisey EE |
| Citation(s) |
25516972 |
| Submission date |
Aug 22, 2014 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Michael Patrick Morley |
| E-mail(s) |
mmorley@pennmedicine.upenn.edu
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| Phone |
215-898-2026
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| Organization name |
Perelman School of Medicine at the University of Pennsylvania
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| Department |
Penn Cardiovascular Institute
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| Street address |
3400 Civic Center Blvd, Bldg 421
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA259208 |
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