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| Status |
Public on Nov 20, 2014 |
| Title |
p63+/Krt5+ Distal Airway Stem Cells are Essential for Lung Regeneration (before and after in vitro differentiation) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The possibility of lung regeneration has been long discounted due to the irreversible nature of chronic lung diseases. However, patients who sustain massive loss of lung tissue during acute infections often recover full pulmonary function. Correspondingly, we previously demonstrated lung regeneration in mice following H1N1 influenza virus infection and implicated p63+Krt5+ distal airway stem cells, or DASCp63/Krt5, in this process. We show here that rare, preexisting DASCp63/K5 undergo a proliferative expansion in response to influenza and lineage-trace to nascent alveoli assembled at sites of interstitial inflammation. We also show that the ablation of DASCp63/Krt5 in vivo prevents the regeneration of lung tissue following influenza leading to pre-fibrotic lesions and deficient oxygen exchange. Finally, we demonstrate that exogenously cloned and propagated DASCp63/Krt5 readily contribute to lung regeneration following transplantation. The transplanted DASC ameliorated influenza-induced lung injury. These data suggest that DASCp63/K5 are required for lung regeneration and may have therapeutic utility in acute and chronic lung diseases.
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| Overall design |
Stem cells before and after in vitro differentiation were subjected to whole genome microarray analysis. Duplicates were included for each sample. We used the Affymetrix Mouse Exon 1.0 ST platform
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| Contributor(s) |
Zuo W, Zhang T, Wu DZ, Guan S, Liew AA, Yamamoto Y, Lim TS, Vicent M, Lessard M, Crum CP, Xian W, McKeon F |
| Citation(s) |
25383540 |
| Submission date |
Aug 27, 2014 |
| Last update date |
Jan 08, 2018 |
| Contact name |
Frank McKeon |
| E-mail(s) |
mckeon.xian@gmail.com
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| Phone |
8608372486
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| Organization name |
University of Houston
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| Department |
Biology and Biochemistry
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| Street address |
3455 Cullen Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE60849 |
p63+/Krt5+ Distal Airway Stem Cells are Essential for Lung Regeneration |
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| Relations |
| BioProject |
PRJNA259662 |
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