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Series GSE6084 Query DataSets for GSE6084
Status Public on Jan 10, 2009
Title Expression data from Murine T cell in response to cycloheximide treatment
Organism Mus musculus
Experiment type Expression profiling by array
Summary Cycloheximide has been used for inhibiting protein synthesis in order to identify IL-2 direct targeted genes in T cells, but the effects of cycloheximide itself on genomewide genes are not well defined
We conducted a global analysis of cycloheximide regulated genes in mouse T cell line CTLL-2 using Affymetrix mouse genome array, and identified 213 genes differentially expressed (?1.5-fold change) in response to cycloheximide 30min after adding cylcoheximide to T cells cultured without IL-2 for 14 hours. These genes involve in a number biological processes.
Keywords: stress response
Overall design Murine cytotoxic T lymphocyte cell line CTLL-2, derived from a C57BL/6 mouse, is an IL-2 dependent cell line. Cells were cultured in D10 medium in an incubator with 5% CO2 at 37oC to mid-log phase (~2 X 105 cells/mL), and collected by centrifugation, washed with phosphate buffered saline (PBS) (Invitrogen), resuspended at 1 X 106 cells/mL in the medium without IL-2 (IL-2 starvation) and cultured for 14 hours (No IL-2 stimulation, T0, 5 reps), followed by adding cycloheximide (20 ?g/mL) for 30min (T4-CHX, 4 reps). Total RNA were extracted from the cells and was hybridized on Affymetrix GeneChip Mouse Genome 430 2.0 Array.
Contributor(s) Zhang Z, Martino A, Faulon J
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Submission date Oct 19, 2006
Last update date Feb 11, 2019
Contact name Zhaoduo Zhang
Phone 925-294-2513
Fax 925-294-3282
Organization name Sandia National Labs
Department Biosystems
Street address 7011 East Ave
City Livermore
State/province CA
ZIP/Postal code 94551
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM108343 T cell_IL-2_Cycloheximide_4 hour_rep1
GSM108344 T cell_IL-2_Cycloheximide_4 hour_rep2
GSM108345 T cell_IL-2_Cycloheximide_4 hour_rep3
BioProject PRJNA97555

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Supplementary file Size Download File type/resource
GSE6084_RAW.tar 40.4 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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