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| Status |
Public on Sep 03, 2014 |
| Title |
Gene expression profile of HoxA9 over expressing and empty vector (EV) control Hemato-Endothelial Progenitors (HEPs) subpopulations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
During hematopoietic differentiation of hESCs, HOXA9 expression parallels hematopoietic development but is restricted to the hemogenic precursors (HEP, CD31+CD34+CD45-), and diminishes as HEPs differentiate into blood cells (CD45+). Enforced expression of Hoxa9 in hESCs robustly promoted differentiation into primitive (CD34+CD45+) and total (CD45+) blood cells with higher clonogenic (CFU) potential. To identify patterns of gene expression that could explain at the molecular level the developmental impact of HOXA9 in hematopoietic commitment of hESCs, we performed gene expression profile in FACS-purified EV- and HOXA9-HEPs.
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| Overall design |
10^5 HoxA9 over expressing and EV control HEPs purified by FACS from H9 and AND1 hEBs at day 15 of hematopoietic differentiation were used for gene expression analysis using Whole Human Genome Oligo Microarray chips (Agilent Technologies).
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| Contributor(s) |
Ramos-Mejia V, Menendez P |
| Citation(s) |
25185710 |
| Submission date |
Sep 02, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Pedro Jose Real |
| E-mail(s) |
pedro.real@genyo.es
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| Phone |
+34 958 71 55 00
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| Organization name |
GENyO
|
| Department |
Molecular Oncology
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| Lab |
Gene Regulation, Stem Cells and Development
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| Street address |
Avda. Ilustracion 114
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| City |
Granada |
| State/province |
Granada |
| ZIP/Postal code |
18016 |
| Country |
Spain |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA260082 |
