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Series GSE6102 Query DataSets for GSE6102
Status Public on Oct 26, 2006
Title Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary We previously reported that lifetime consumption of soy proteins or whey proteins reduced the incidence of azoxymethane (AOM)-induced colon tumors in rats. To obtain insights into these effects, global gene expression profiles of colons from rats with lifetime ingestion of casein (CAS, control diet), soy protein isolate (SPI), and whey protein hydrolysate (WPH) diets were determined. We identified 31 induced and 49 repressed genes in the proximal colons of the SPI-fed group and 44 induced and 119 repressed genes in the proximal colons of the WPH-fed group, relative to CAS. Hierarchical clustering identified the co-induction or co-repression of multiple genes by SPI and WPH. The differential expression of I-FABP (2.92-, 3.97-fold down-regulated in SPI and WPH fed rats; P = 0.023, P = 0.01, respectively), cyclin D1 (1.61-, 2.42-fold down-regulated in SPI and WPH fed rats; P = 0.033, P = 0.001, respectively), and the c-neu proto-oncogene (2.46-, 4.10-fold down-regulated in SPI and WPH fed rats; P < 0.001, P < 0.001, respectively) mRNAs were confirmed by real-time quantitative RT-PCR. SPI and WPH affected colonic neuro-endocrine gene expression: peptide YY (PYY) and glucagon mRNAs were down-regulated in WPH fed rats, whereas somatostatin mRNA and corresponding circulating protein levels, were enhanced by SPI and WPH. The identification of transcripts co- or differentially-regulated by SPI and WPH diets suggests common as well as unique anti-tumorigenesis mechanisms of action which may involve growth factor, neuroendocrine and immune system genes. SPI and WPH induction of somatostatin, a known anti-proliferative agent for colon cancer cells, would inhibit tumorigenesis
Keywords: Comarative genomic hybridization
 
Overall design Male Sprague Dawley rats, fed one of the three purified diets, were studied at 40 weeks after AOM injection and when tumors had developed in some animals of each group. Total RNA, purified from non-tumor tissue within the proximal half of each colon, was used to prepare biotinylated probes, which were hybridized to Affymetrix RG_U34A rat microarrays.
 
Contributor(s) Xiao R, Badger TM, Simmen FA
Citation(s) 15644144
Submission date Oct 23, 2006
Last update date Feb 21, 2017
Contact name Rijin Xiao
E-mail(s) xiaorijin@uams.edu
Phone 501-364-2790
Fax 501-364-3161
Organization name Nutrition Center, Arkansas Chidren's Hospital
Street address 2020 Marshall Street
City Little Rock
State/province AR
ZIP/Postal code 72202
Country USA
 
Platforms (1)
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
Samples (9)
GSM141501 Proximal colon casein 1
GSM141502 Proximal colon casein 2
GSM141503 Proximal colon casein 3
Relations
BioProject PRJNA97665

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Supplementary file Size Download File type/resource
GSE6102_RAW.tar 17.2 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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